GeneTex

  • Country / Location Selection

United States (US)

ABCA1 antibody

Orthogonal
Anti-ABCA1 antibody used in Western Blot (WB). GTX27360
Anti-ABCA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX27360
Anti-ABCA1 antibody used in Western Blot (WB). GTX27360
Anti-ABCA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27360
Anti-ABCA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27360
Anti-ABCA1 antibody used in Western Blot (WB). GTX27360
Anti-ABCA1 antibody used in Flow cytometry (FACS). GTX27360

Cat. No. GTX27360

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, ChIP assay, Gel supershift assays

Reactivity

 Human, Mouse, Rat, Dog, Hamster, Chicken, Pig, Mustelid
Package
100 μl ($449)
See all ABCA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:100
IHC-P 1:200
IHC-Fr Assay dependent
FACS 1:400
IP 1:10 - 1:500
ELISA Assay dependent
ChIP assay Assay dependent
Gel supershift assays Assay dependent
Not tested in other applications.

Calculated MW

254 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Partial peptide sequence (within residues 1100-1300) of human ABCA1 [UniProt# O95477]. Actual immunogen sequence is proprietary information.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_367201

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP binding cassette subfamily A member 1 , ABC-1 , ABC1 , CERP , HDLDT1 , TGD

Cellular Localization

Membrane

Background

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency. [provided by RefSeq]

Database

  • Gene ID: 19 ABCA1
  • UniProt: O95477 ABCA1

Research Area

DATA IMAGES

Anti-ABCA1 antibody used in Western Blot (WB). GTX27360

GTX27360 WB Image

WB analysis of mouse peritoneal macrophages using GTX27360 ABCA1 antibody.
Lane 1 : T09 cell lysate
Lane 2 : Induced T09 cell lysate

Anti-ABCA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX27360

GTX27360 IHC-P Image

IHC-P analysis of prostate tissue using GTX27360 ABCA1 antibody.

Anti-ABCA1 antibody used in Western Blot (WB). GTX27360

GTX27360 WB Image

Orthogonal

WB analysis of RAW264.7 cells treated with vehicle (-) or 9-cisretinoic acid and 22rhydroxycholesterol (+) using GTX27360 ABCA1 antibody.
Loading : 40μg

Anti-ABCA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27360

GTX27360 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX27360 ABCA1 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 5 μg/ml
Fixation : 10% formalin
Permibilization : 0.5% Triton-X100

Anti-ABCA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27360

GTX27360 ICC/IF Image

ICC/IF analysis of HepG2 cells(serum starvation 24hrs and treated with 1uM TO9) using GTX27360 ABCA1 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 5 μg/ml
Fixation : 10% formalin
Permibilization : 1X TBS + 0.5% Triton-X100 for 5 minutes

Anti-ABCA1 antibody used in Western Blot (WB). GTX27360

GTX27360 WB Image

WB analysis of post-nuclear lysate of raw macrophages treated with 9-cisretinoic acid and 22R-hydroxycholesterol using GTX27360 ABCA1 antibody.
Loading : 40μg

Anti-ABCA1 antibody used in Flow cytometry (FACS). GTX27360

GTX27360 FACS Image

FACS analysis of HeLa cells using GTX27360 ABCA1 antibody. M1 is defined by unstained cells.
Purple : primary antibody
Dilution : 1:400

REFERENCE

Application Reference

Wang SM et al. Cell Death Discov 2021; 7 (1) : 8 CCAAT/Enhancer-binding protein delta mediates glioma stem-like cell enrichment and ATP-binding cassette transporter ABCA1 activation for temozolomide resistance in glioblastoma.
Application : WB
Reactivity : Human
Chan HC et al. Biomedicines 2020; 8 (12) An Increased Plasma Level of ApoCIII-Rich Electronegative High-Density Lipoprotein May Contribute to Cognitive Impairment in Alzheimer's Disease.
Application : WB
Reactivity : Mouse
Yeh YT et al. Nutrients 2017; 9 (10) Chinese Olive (Canarium album L.) Fruit Extract Attenuates Metabolic Dysfunction in Diabetic Rats.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

There are currently no reviews for ABCA1 antibody (GTX27360). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 449