GeneTex
United States (US)

ABCB5 antibody [5H3C6]

GTX60661_20170912_ELISA_18121411_511.jpg
GTX60661_20170912_ICCIF_18121411_657.jpg
GTX60661_20170912_IHC-P_18121411_433.jpg
GTX60661_20170912_IHC-P_1_18121411_306.jpg
GTX60661_20170912_FACS_18121411_561.jpg
GTX60661_20170912_WB_18121411_314.jpg

Cat No. GTX60661

Host Mouse
Clonality Monoclonal
Clone Name 5H3C6
Isotype IgG1
Application WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

90 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human ABCB5 (AA: 481-674) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

EST422562 Antibody , ABCB5BETA Antibody , ABCB5ALPHA Antibody

Background

ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES
GTX60661_20170912_ELISA_18121411_511.jpg

GTX60661 ELISA Image

ELISA analysis of antigen using GTX60661 ABCB5 antibody [5H3C6].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

GTX60661_20170912_ICCIF_18121411_657.jpg

GTX60661 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60661 ABCB5 antibody [5H3C6].
Green : ABCB5
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

GTX60661_20170912_IHC-P_18121411_433.jpg

GTX60661 IHC-P Image

IHC-P analysis of breast cancer tissue using GTX60661 ABCB5 antibody [5H3C6].

GTX60661_20170912_IHC-P_1_18121411_306.jpg

GTX60661 IHC-P Image

IHC-P analysis of liver cancer tissue using GTX60661 ABCB5 antibody [5H3C6].

GTX60661_20170912_FACS_18121411_561.jpg

GTX60661 FACS Image

FACS analysis of HepG2 cells using GTX60661 ABCB5 antibody [5H3C6].
Green : ABCB5
Purple : negative control

GTX60661_20170912_WB_18121411_314.jpg

GTX60661 WB Image

WB analysis of A431 cell lysate using GTX60661 ABCB5 antibody [5H3C6].

REFERENCE
Package List Price ($)
$ 319