GeneTex
United States (US)

ABCB5 antibody [5H3C6]

GTX60661_20170912_FACS_18121411_561.jpg
GTX60661_20170912_WB_18121411_314.jpg
GTX60661_20170912_ELISA_18121411_511.jpg
GTX60661_20170912_ICCIF_18121411_657.jpg
GTX60661_20170912_IHC-P_18121411_433.jpg
GTX60661_20170912_IHC-P_1_18121411_306.jpg

Cat No. GTX60661

Host Mouse
Clonality Monoclonal
Clone Name 5H3C6
Isotype IgG1
Application WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

90 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human ABCB5 (AA: 481-674) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Atp Binding Cassette Subfamily B Member 5,Abcb5Alpha,Abcb5Beta,Est422562,Abcb5

Background

ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES
GTX60661_20170912_FACS_18121411_561.jpg

GTX60661 FACS Image

FACS analysis of HepG2 cells using GTX60661 ABCB5 antibody [5H3C6].
Green : ABCB5
Purple : negative control

GTX60661_20170912_WB_18121411_314.jpg

GTX60661 WB Image

WB analysis of A431 cell lysate using GTX60661 ABCB5 antibody [5H3C6].

GTX60661_20170912_ELISA_18121411_511.jpg

GTX60661 ELISA Image

ELISA analysis of antigen using GTX60661 ABCB5 antibody [5H3C6].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

GTX60661_20170912_ICCIF_18121411_657.jpg

GTX60661 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60661 ABCB5 antibody [5H3C6].
Green : ABCB5
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

GTX60661_20170912_IHC-P_18121411_433.jpg

GTX60661 IHC-P Image

IHC-P analysis of breast cancer tissue using GTX60661 ABCB5 antibody [5H3C6].

GTX60661_20170912_IHC-P_1_18121411_306.jpg

GTX60661 IHC-P Image

IHC-P analysis of liver cancer tissue using GTX60661 ABCB5 antibody [5H3C6].

REFERENCE
Package List Price ($)
$ 319