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ABCG1 antibody [GT1330]

Recombinant antibody
Anti-ABCG1 antibody [GT1330] used in Western Blot (WB). GTX03242
Anti-ABCG1 antibody [GT1330] used in Western Blot (WB). GTX03242
Anti-ABCG1 antibody [GT1330] used in Western Blot (WB). GTX03242

Cat. No. GTX03242

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1330

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($439)
See all ABCG1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Observed MW

110 kDa. The observed M.W. is based on the following publication. PMID: 31003090

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human ABCG1.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP binding cassette subfamily G member 1 , ABC8 , WHITE1

Cellular Localization

Endoplasmic reticulum membrane , Golgi apparatus membrane

Background

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types. Six alternative splice variants have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ABCG1 antibody [GT1330] used in Western Blot (WB). GTX03242

GTX03242 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ABCG1 antibody [GT1330] (GTX03242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 31003090

Anti-ABCG1 antibody [GT1330] used in Western Blot (WB). GTX03242

GTX03242 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ABCG1 antibody [GT1330] (GTX03242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 31003090

Anti-ABCG1 antibody [GT1330] used in Western Blot (WB). GTX03242

GTX03242 WB Image

WB analysis of various samples using GTX03242 ABCG1 antibody [GT1330].
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

Application Reference

Wang SA et al. Mol Oncol 2023; NCI677397 targeting USP24-mediated induction of lipid peroxidation induces ferroptosis in drug-resistant cancer cells
Application : WB
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REVIEW

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Package List Price ($)
$ 439