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ABCG2 antibody [3G8]

Anti-ABCG2 antibody [3G8] used in ELISA (ELISA). GTX60447
Anti-ABCG2 antibody [3G8] used in Flow cytometry (FACS). GTX60447
Anti-ABCG2 antibody [3G8] used in IHC (Paraffin sections) (IHC-P). GTX60447
Anti-ABCG2 antibody [3G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60447
Anti-ABCG2 antibody [3G8] used in Western Blot (WB). GTX60447

Cat. No. GTX60447

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3G8

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Monkey
Package
100 μl ($399)
See all ABCG2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human ABCG2 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_2887969

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP binding cassette subfamily G member 2 (Junior blood group) , ABC15 , ABCP , BCRP , BCRP1 , BMDP , CD338 , CDw338 , EST157481 , GOUT1 , MRX , MXR , MXR-1 , MXR1 , UAQTL1

Cellular Localization

Cell membrane

Background

The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Database

Research Area

DATA IMAGES

Anti-ABCG2 antibody [3G8] used in ELISA (ELISA). GTX60447

GTX60447 ELISA Image

ELISA analysis of antigen using GTX60447 ABCG2 antibody [3G8].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-ABCG2 antibody [3G8] used in Flow cytometry (FACS). GTX60447

GTX60447 FACS Image

FACS analysis of HepG2 cells using GTX60447 ABCG2 antibody [3G8].
Green : ABCG2
Purple : negative control

Anti-ABCG2 antibody [3G8] used in IHC (Paraffin sections) (IHC-P). GTX60447

GTX60447 IHC-P Image

IHC-P analysis of bladder cancer tissue (left) and skeletal muscle tissue (right) using GTX60447 ABCG2 antibody [3G8].

Anti-ABCG2 antibody [3G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60447

GTX60447 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60447 ABCG2 antibody [3G8].
Green : ABCG2
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-ABCG2 antibody [3G8] used in Western Blot (WB). GTX60447

GTX60447 WB Image

WB analysis of NIH3T3 (1) and Cos7 (2) cell lysate using GTX60447 ABCG2 antibody [3G8].

REFERENCE

Application Reference

Zhang W et al. Molecules 2019; 24 (3) P-glycoprotein Inhibitor Tariquidar Potentiates Efficacy of Astragaloside IV in Experimental Autoimmune Encephalomyelitis Mice.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399