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ABCG2 antibody

Anti-ABCG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100437
Anti-ABCG2 antibody used in Western Blot (WB). GTX100437
Anti-ABCG2 antibody used in Western Blot (WB). GTX100437

Cat No. GTX100437

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , NIH-3T3

Predict Reactivity

Rat, Sheep, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ABCG2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ATP binding cassette subfamily G member 2 (Junior blood group),ABC15,ABCP,BCRP,BCRP1,BMDP,CD338,CDw338,EST157481,GOUT1,MRX,MXR,MXR-1,MXR1,UAQTL1

Cellular Localization

Cell membrane

Background

The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-ABCG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100437

GTX100437 IHC-P Image

ABCG2 antibody detects ABCG2 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: ABCG2 antibody (GTX100437) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ABCG2 antibody used in Western Blot (WB). GTX100437

GTX100437 WB Image

Sample (30 µg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX100437 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ABCG2 antibody used in Western Blot (WB). GTX100437

GTX100437 WB Image

ABCG2 antibody detects ABCG2 protein by western blot analysis.
A. 30 µg A549 whole cell lysate/extract
B. 30 µg H1299 whole cell lysate/extract
C. 30 µg HCT116 whole cell lysate/extract
7.5% SDS-PAGE
ABCG2 antibody (GTX100437) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169