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ABCG2 antibody

Anti-ABCG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100437
Anti-ABCG2 antibody used in Western Blot (WB). GTX100437
Anti-ABCG2 antibody used in Western Blot (WB). GTX100437
Anti-ABCG2 antibody used in Western Blot (WB). GTX100437

Cat. No. GTX100437

Reference 12

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ABCG2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , NIH-3T3

Predict Reactivity

Rat, Sheep, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human ABCG2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240399

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP binding cassette subfamily G member 2 (Junior blood group) , ABC15 , ABCP , BCRP , BCRP1 , BMDP , CD338 , CDw338 , EST157481 , GOUT1 , MRX , MXR , MXR-1 , MXR1 , UAQTL1

Cellular Localization

Cell membrane

Background

The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ABCG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100437

GTX100437 IHC-P Image

ABCG2 antibody detects ABCG2 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: ABCG2 antibody (GTX100437) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ABCG2 antibody used in Western Blot (WB). GTX100437

GTX100437 WB Image

Sample (30 μg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX100437 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ABCG2 antibody used in Western Blot (WB). GTX100437

GTX100437 WB Image

ABCG2 antibody detects ABCG2 protein by western blot analysis.
A. 30 μg A549 whole cell lysate/extract
B. 30 μg H1299 whole cell lysate/extract
C. 30 μg HCT116 whole cell lysate/extract
7.5% SDS-PAGE
ABCG2 antibody (GTX100437) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ABCG2 antibody used in Western Blot (WB). GTX100437

GTX100437 WB Image

The data was published in the journal Sci Rep in 2018. PMID: 30310131

REFERENCE

Application Reference

Chen J et al. Stem Cell Res Ther 2024; 15 (1) : 16 Human intestinal organoid-derived PDGFRα?+?mesenchymal stroma enables proliferation and maintenance of LGR4?+?epithelial stem cells.
Seung-Hun Kim et al. Cancer Drug Resist 2021; 4 (4) : 866-880 The aqueous extract of brucea javanica reduces tumorigenicity of human lung cancer tumorspheres.
Application : WB
Reactivity : Human
Shahnawaz S et al. Antioxidants (Basel) 2021; 10 (11) Dysregulated Autophagy Leads to Oxidative Stress and Aberrant Expression of ABC Transporters in Women with Early Miscarriage.
Application : WB
Reactivity : Human
Onuma S et al. Cells 2021; 10 (5) Upregulation of Chemoresistance by Mg2+ Deficiency through Elevation of ATP Binding Cassette Subfamily B Member 1 Expression in Human Lung Adenocarcinoma A549 Cells.
Application : WB
Reactivity : Human
Biswas S et al. Asian Pac J Cancer Prev 2021; 22 (3) : 957-970 Curcumin Rescues Doxorubicin Responsiveness via Regulating Aurora a Signaling Network in Breast Cancer Cells.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Chen J et al. Stem Cell Res Ther 2024; 15 (1):16 Human intestinal organoid-derived PDGFRα?+?mesenchymal stroma enables proliferation and maintenance of LGR4?+?epithelial stem cells.
Seung-Hun Kim et al. Cancer Drug Resist 2021; 4 (4):866-880 The aqueous extract of brucea javanica reduces tumorigenicity of human lung cancer tumorspheres.
Application : WB
Reactivity : Human
Shahnawaz S et al. Antioxidants (Basel) 2021; 10 (11) Dysregulated Autophagy Leads to Oxidative Stress and Aberrant Expression of ABC Transporters in Women with Early Miscarriage.
Application : WB
Reactivity : Human
Onuma S et al. Cells 2021; 10 (5) Upregulation of Chemoresistance by Mg2+ Deficiency through Elevation of ATP Binding Cassette Subfamily B Member 1 Expression in Human Lung Adenocarcinoma A549 Cells.
Application : WB
Reactivity : Human
Biswas S et al. Asian Pac J Cancer Prev 2021; 22 (3):957-970 Curcumin Rescues Doxorubicin Responsiveness via Regulating Aurora a Signaling Network in Breast Cancer Cells.
Application : WB
Reactivity : Human
Wang SA et al. Cell Death Differ 2021; (Epub) USP24 promotes drug resistance during cancer therapy.
Application : WB
Reactivity : Human
Fang CH et al. J Biomed Sci 2020; 27 (1):42 A novel c-Kit/phospho-prohibitin axis enhances ovarian cancer stemness and chemoresistance via Notch3-PBX1 and 帣-catenin-ABCG2 signaling.
Application : WB
Reactivity : Human
Maruhashi R et al. Sci Rep 2019; 9 (1):13753 Chrysin enhances anticancer drug-induced toxicity mediated by the reduction of claudin-1 and 11 expression in a spheroid culture model of lung squamous cell carcinoma cells.
Application : WB
Reactivity : Human
Ma L et al. Lung Cancer Manag 2019; 8 (1):LMT04 Targeting claudin-3 suppresses stem cell-like phenotype in nonsquamous non-small-cell lung carcinoma.
Eguchi H et al. Sci Rep 2018; 8 (1):15157 Increase in resistance to anticancer drugs involves occludin in spheroid culture model of lung adenocarcinoma A549 cells.
Application : WB
Reactivity : Human
Maruhashi R et al. Biochim Biophys Acta 2017; 1865 (3):470-479 Elevation of sensitivity to anticancer agents of human lung adenocarcinoma A549 cells by knockdown of claudin-2 expression in monolayer and spheroid culture models.
Application : WB
Reactivity : Human
Ni YL et al. Apoptosis 2017; (Epub) A potent indolylquinoline alleviates growth of human lung cancer cell tumorspheres.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169