APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.3-1μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88640-PEP).
Product Note
This antibody is expected to recognize isoform a (NP_001035937.1) only.
Predict Reactivity
Human(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-REFLGKSEPEHQH, from the C Terminus of the protein sequence according to NP_001035937.1; NP_056415.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
abhydrolase domain containing 12 , ABHD12A , BEM46L2 , C20orf22 , PHARC , dJ965G21.2
Cellular Localization
Membrane
Background
This gene encodes an enzyme that catalyzes the hydrolysis of 2-arachidonoyl glycerol (2-AG), the main endocannabinoid lipid transmitter that acts on cannabinoid receptors, CB1 and CB2. The endocannabinoid system is involved in a wide range of physiological processes, including neurotransmission, mood, appetite, pain appreciation, addiction behavior, and inflammation. Mutations in this gene are associated with the neurodegenerative disease, PHARC (polyneuropathy, hearing loss, ataxia, retinitis pigmentosa, and cataract), resulting from an inborn error of endocannabinoid metabolism. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jan 2011]
Database
Research Area
DATA IMAGES
|
GTX88640 WB Image
WB analysis of mouse brain lysate using GTX88640 ABHD12 antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
|
REFERENCE
There are currently no references for ABHD12 antibody, C-term (GTX88640). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ABHD12 antibody, C-term (GTX88640). Be the first to share your experience with this product.
