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ABHD5 antibody, Internal

Anti-ABHD5 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89147
Anti-ABHD5 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89147
Anti-ABHD5 antibody, Internal used in Western Blot (WB). GTX89147

Cat. No. GTX89147

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all ABHD5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-0.6μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89147-PEP).

Product Note

This antibody is not expected to cross-react with ABHD4.

Predict Reactivity

Rat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-FPERPDLADQDR, from the internal region of the protein sequence according to NP_057090.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

abhydrolase domain containing 5 , CGI58 , IECN2 , NCIE2

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ABHD5 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89147

GTX89147 IHC-P Image

IHC-P analysis of human kidney using GTX89147 ABHD5 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

Anti-ABHD5 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89147

GTX89147 IHC-P Image

IHC-P analysis of human pancreas using GTX89147 ABHD5 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

Anti-ABHD5 antibody, Internal used in Western Blot (WB). GTX89147

GTX89147 WB Image

WB analysis of NIH-3T3 lysate using GTX89147 ABHD5 antibody, Internal.
Dilution : 0.2μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399