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ABI3 antibody

Anti-ABI3 antibody used in Western Blot (WB). GTX60228
Anti-ABI3 antibody used in Western Blot (WB). GTX60228
Anti-ABI3 antibody used in IHC (Paraffin sections) (IHC-P). GTX60228
Anti-ABI3 antibody used in Western Blot (WB). GTX60228

Cat. No. GTX60228

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all ABI3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:50-400
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide derived from human ABI3(140-190).

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_2887958

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ABI family member 3 , NESH , SSH3BP3

Cellular Localization

Cytoplasm

Background

This gene encodes a member of an adaptor protein family. Members of this family encode proteins containing a homeobox homology domain, proline rich region and Src-homology 3 (SH3) domain, and are components of the Abi/WAVE complex which regulates actin polymerization. The encoded protein inhibits ectopic metastasis of tumor cells as well as cell migration. This may be accomplished through interaction with p21-activated kinase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Database

Research Area

DATA IMAGES

Anti-ABI3 antibody used in Western Blot (WB). GTX60228

GTX60228 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with ABI3 antibody (GTX60228) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ABI3 antibody used in Western Blot (WB). GTX60228

GTX60228 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with ABI3 antibody (GTX60228) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ABI3 antibody used in IHC (Paraffin sections) (IHC-P). GTX60228

GTX60228 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX60228 ABI3 antibody.
Dilution : 1:200

Anti-ABI3 antibody used in Western Blot (WB). GTX60228

GTX60228 WB Image

The data was published in the journal Cell Commun Signal in 2019. PMID: 31530281

REFERENCE

Application Reference

Nath D et al. Cell Commun Signal 2019; 17 (1) : 120 Abi1 loss drives prostate tumorigenesis through activation of EMT and non-canonical WNT signaling.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399