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ABL2 antibody [N1N3]

KO/KD
Anti-ABL2 antibody [N1N3] used in Western Blot (WB). GTX103584

Cat No. GTX103584

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all ABL2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

128 kDa. ( Note )

Positive Control

IMR32

Specificity/Sensitivity

Knockdown/Knockout validation was supported by references (PMID:27293031)

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ABL2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ABL proto-oncogene 2, non-receptor tyrosine kinase , ABLL , ARG

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes a member of the Abelson family of nonreceptor tyrosine protein kinase. The protein is highly similar to the ABL1 protein, including the tyrosine kinase, SH2 and SH3 domains, and has a role in cytoskeletal rearrangements by its C-terminal F-actin- and microtubule-binding sequences. This gene is expressed in both normal and tumor cells, and is involved in translocation with the ETV6 gene in leukemia. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ABL2 antibody [N1N3] used in Western Blot (WB). GTX103584

GTX103584 WB Image

Sample (30 μg of whole cell lysate)
A: IMR32
5% SDS PAGE
GTX103584 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Frolov A et al. Sci Rep 2016; 6 : 27378 Imatinib and * increase glioblastoma cell invasion via Abl-independent stimulation of p130Cas and FAK signalling.
Application : WB
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169