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ACAA1 antibody

Anti-ACAA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114229
Anti-ACAA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114229
Anti-ACAA1 antibody used in Western Blot (WB). GTX114229
Anti-ACAA1 antibody used in Western Blot (WB). GTX114229

Cat No. GTX114229

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

A549 , HeLa , mouse Liver

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACAA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620789

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

acetyl-CoA acyltransferase 1 , ACAA , PTHIO , THIO

Cellular Localization

Peroxisome

Background

This gene encodes an enzyme operative in the beta-oxidation system of the peroxisomes. Deficiency of this enzyme leads to pseudo-Zellweger syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

  • Gene ID: 30 ACAA1
  • UniProt: P09110 ACAA1

Research Area

DATA IMAGES

Anti-ACAA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114229

GTX114229 ICC/IF Image

ACAA1 antibody detects ACAA1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ACAA1 protein stained by ACAA1 antibody (GTX114229) diluted at 1:250.
Blue: Hoechst 33342 staining.

Anti-ACAA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114229

GTX114229 IHC-P Image

ACAA1 antibody detects ACAA1 protein at cytosol on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
ACAA1 antibody (GTX114229) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ACAA1 antibody used in Western Blot (WB). GTX114229

GTX114229 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: Hela
10% SDS PAGE
GTX114229 diluted at 1:1000

Anti-ACAA1 antibody used in Western Blot (WB). GTX114229

GTX114229 WB Image

Sample (50 ug of whole cell lysate)
A: mouse Liver
10% SDS PAGE
GTX114229 diluted at 1:1000

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169