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ACAA1 antibody

Anti-ACAA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114229
Anti-ACAA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114229
Anti-ACAA1 antibody used in Western Blot (WB). GTX114229
Anti-ACAA1 antibody used in Western Blot (WB). GTX114229

Cat. No. GTX114229

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ACAA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

mouse Liver , A549 , HeLa

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACAA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620789

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acetyl-CoA acyltransferase 1 , ACAA , PTHIO , THIO

Cellular Localization

Peroxisome

Background

This gene encodes an enzyme operative in the beta-oxidation system of the peroxisomes. Deficiency of this enzyme leads to pseudo-Zellweger syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

  • Gene ID: 30 ACAA1
  • UniProt: P09110 ACAA1

Research Area

DATA IMAGES

Anti-ACAA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114229

GTX114229 ICC/IF Image

ACAA1 antibody detects ACAA1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ACAA1 protein stained by ACAA1 antibody (GTX114229) diluted at 1:250.
Blue: Hoechst 33342 staining.
Anti-ACAA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114229

GTX114229 IHC-P Image

ACAA1 antibody detects ACAA1 protein at cytosol on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
ACAA1 antibody (GTX114229) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ACAA1 antibody used in Western Blot (WB). GTX114229

GTX114229 WB Image

Sample (50 ug of whole cell lysate)
A: mouse Liver
10% SDS PAGE
GTX114229 diluted at 1:1000

Anti-ACAA1 antibody used in Western Blot (WB). GTX114229

GTX114229 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: Hela
10% SDS PAGE
GTX114229 diluted at 1:1000

REFERENCE

Application Reference

Mark Li et al. Mol Ther Nucleic Acids 2021; 26 : 307-320 A putative long noncoding RNA-encoded micropeptide maintains cellular homeostasis in pancreatic β cells.
Application : ICC/IF
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169