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ACAA1 antibody

Anti-ACAA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114230
Anti-ACAA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114230
Anti-ACAA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114230
Anti-ACAA1 antibody used in Western Blot (WB). GTX114230

Cat No. GTX114230

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACAA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619766

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

acetyl-CoA acyltransferase 1 , ACAA , PTHIO , THIO

Cellular Localization

Peroxisome

Background

This gene encodes an enzyme operative in the beta-oxidation system of the peroxisomes. Deficiency of this enzyme leads to pseudo-Zellweger syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

  • Gene ID: 30 ACAA1
  • UniProt: P09110 ACAA1

Research Area

DATA IMAGES

Anti-ACAA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114230

GTX114230 IHC-P Image

ACAA1 antibody detects ACAA1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ACAA1 stained by ACAA1 antibody (GTX114230) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACAA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114230

GTX114230 ICC/IF Image

ACAA1 antibody detects ACAA1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ACAA1 protein stained by ACAA1 antibody (GTX114230) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-ACAA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114230

GTX114230 ICC/IF Image

ACAA1 antibody detects ACAA1 protein at Peroxisome by immunofluorescent analysis.
Sample: HepG2 cells were fixed in -20ºC 100% MeOH for 5 min.
Green: ACAA1 protein stained by ACAA1 antibody (GTX114230) diluted at 1:500.
Blue: Hoechst 33343 staining.

Anti-ACAA1 antibody used in Western Blot (WB). GTX114230

GTX114230 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
ACAA1 antibody
GTX114230 diluted at 1:1000

REFERENCE

There are currently no references for ACAA1 antibody (GTX114230). Be the first to share your publications with this product.

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169