GeneTex
United States (US)

ACAA2 antibody

GTX115417_41248_IHC_M_2_18121410_119.jpg
GTX115417_41248_20170913_IFA_18121410_137.jpg
GTX115417_41248_IHC_M_18121410_470.jpg
GTX115417_41248_WB_18121410_688.jpg

Cat No. GTX115417

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
See all ACAA2 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACAA2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Acetyl-Coa Acyltransferase 2,Dsaec,Acaa2

Cellular Localization

Mitochondrion

Background

The encoded protein catalyzes the last step of the mitochondrial fatty acid beta-oxidation spiral. Unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115417_41248_IHC_M_2_18121410_119.jpg

GTX115417 IHC-P Image

ACAA2 antibody detects ACAA2 protein at mitochondria on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
ACAA2 antibody (GTX115417) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX115417_41248_20170913_IFA_18121410_137.jpg

GTX115417 ICC/IF Image

ACAA2 antibody detects ACAA2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ACAA2 protein stained by ACAA2 antibody (GTX115417) diluted at 1:500.
Blue: Hoechst 33342 staining.
GTX115417_41248_IHC_M_18121410_470.jpg

GTX115417 IHC-P Image

ACAA2 antibody detects ACAA2 protein at mitochondria on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ACAA2 antibody (GTX115417) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX115417_41248_WB_18121410_688.jpg

GTX115417 WB Image

ACAA2 antibody detects ACAA2 protein by Western blot analysis.
A. 30 µg HeLa whole cell lysate/extract
10 % SDS-PAGE
ACAA2 antibody (GTX115417) dilution: 1:1000
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169