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ACAA2 antibody

Anti-ACAA2 antibody used in IHC (Paraffin sections) (IHC-P). GTX115417
Anti-ACAA2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115417
Anti-ACAA2 antibody used in IHC (Paraffin sections) (IHC-P). GTX115417
Anti-ACAA2 antibody used in Western Blot (WB). GTX115417

Cat. No. GTX115417

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ACAA2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACAA2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885361

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

acetyl-CoA acyltransferase 2 , DSAEC

Cellular Localization

Mitochondrion

Background

The encoded protein catalyzes the last step of the mitochondrial fatty acid beta-oxidation spiral. Unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ACAA2 antibody used in IHC (Paraffin sections) (IHC-P). GTX115417

GTX115417 IHC-P Image

ACAA2 antibody detects ACAA2 protein at mitochondria on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
ACAA2 antibody (GTX115417) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ACAA2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115417

GTX115417 ICC/IF Image

ACAA2 antibody detects ACAA2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ACAA2 protein stained by ACAA2 antibody (GTX115417) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ACAA2 antibody used in IHC (Paraffin sections) (IHC-P). GTX115417

GTX115417 IHC-P Image

ACAA2 antibody detects ACAA2 protein at mitochondria on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
ACAA2 antibody (GTX115417) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ACAA2 antibody used in Western Blot (WB). GTX115417

GTX115417 WB Image

ACAA2 antibody detects ACAA2 protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
10 % SDS-PAGE
ACAA2 antibody (GTX115417) dilution: 1:1000

REFERENCE

Application Reference

Marco Rosina et al. Cell Metab 2022; 34 (4) : 533-548.e12 Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue.
Application : WB
Reactivity : Mouse
Wang W et al. Biochem Biophys Res Commun 2021; 576 : 15-21 ACAA2 is a ligand-dependent coactivator for thyroid hormone receptor β1.
Application : WB
Reactivity : Mouse
Wei Zhong Zhu et al. Proteome Sci 2020; 18 (1) : 11 Sex impacts cardiac function and the proteome response to thyroid hormone in aged mice.
Application : WB
Reactivity : Mouse
Wei Zhong Zhu et al. Proteome Science 2020; (Epub) Sex Impacts Cardiac Function and the Proteome Response to Thyroid Hormone in Aged Mice.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169