GeneTex
United States (US)

ACADL antibody [N1N2], N-term

GTX100486_40730_WB_19011506_391.jpg
GTX100486_40730_IHC_19011506_255.jpg

Cat No. GTX100486

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

H1299 , HCT116

Predict Reactivity

Mouse, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ACADL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ACAD4 antibody, FLJ94052 antibody, LCAD antibody, ACADL antibody, "acyl-Coenzyme A dehydrogenase, long chain antibody", "long-chain specific acyl-CoA dehydrogenase, mitochondrial antibody", "acyl-CoA dehydrogenase, long chain antibody"

Cellular Localization

Mitochondrion matrix

Background

The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family, which is a family of mitochondrial flavoenzymes involved in fatty acid and branched chain amino-acid metabolism. This protein is one of the four enzymes that catalyze the initial step of mitochondrial beta-oxidation of straight-chain fatty acid. Defects in this gene are the cause of long-chain acyl-CoA dehydrogenase (LCAD) deficiency, leading to nonketotic hypoglycemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100486_40730_WB_19011506_391.jpg

GTX100486 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: HCT116
10% SDS PAGE
GTX100486 diluted at 1:1000

GTX100486_40730_IHC_19011506_255.jpg

GTX100486 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using ACADL(GTX100486) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169