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ACADL antibody [N1N2], N-term

Anti-ACADL antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100486
Anti-ACADL antibody [N1N2], N-term used in Western Blot (WB). GTX100486

Cat. No. GTX100486

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ACADL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

H1299 , HCT116

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ACADL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173327

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acyl-CoA dehydrogenase long chain , ACAD4 , LCAD

Cellular Localization

Mitochondrion matrix

Background

The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family, which is a family of mitochondrial flavoenzymes involved in fatty acid and branched chain amino-acid metabolism. This protein is one of the four enzymes that catalyze the initial step of mitochondrial beta-oxidation of straight-chain fatty acid. Defects in this gene are the cause of long-chain acyl-CoA dehydrogenase (LCAD) deficiency, leading to nonketotic hypoglycemia. [provided by RefSeq]

Database

  • Gene ID: 33 ACADL
  • UniProt: P28330 ACADL

Research Area

DATA IMAGES

Anti-ACADL antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100486

GTX100486 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using ACADL(GTX100486) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ACADL antibody [N1N2], N-term used in Western Blot (WB). GTX100486

GTX100486 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: HCT116
10% SDS PAGE
GTX100486 diluted at 1:1000

REFERENCE

Application Reference

Tenopoulou M et al. J Biol Chem 2015; 290 (16) : 10486-94 Strategies for correcting very long chain acyl-CoA dehydrogenase deficiency.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169