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ACADM antibody, C-term

Anti-ACADM antibody, C-term used in Western Blot (WB). GTX13677
Anti-ACADM antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX13677

Cat. No. GTX13677

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all ACADM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.01-0.1μg/ml
IHC-P 3-5μg/ml

Note :

IHC-P
Human Liver shows mitochondrial staining in hepatocytes.
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX13677-PEP).

Product Note

This antibody is expected to recognise both reported isoforms (NP_000007.1; NP_001120800.1).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-RLIVAREHIDKYKN, from the C Terminus of the protein sequence according to NP_000007.1; NP_001120800.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acyl-CoA dehydrogenase medium chain , ACAD1 , MCAD , MCADH

Cellular Localization

Mitochondrion matrix

Background

This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

  • Gene ID: 34 ACADM
  • UniProt: P11310 ACADM

Research Area

DATA IMAGES

Anti-ACADM antibody, C-term used in Western Blot (WB). GTX13677

GTX13677 WB Image

WB analysis of human heart lysate using GTX13677 ACADM antibody, C-term.
Dilution : 0.05μg/ml
Loading : 35μg protein in RIPA buffer

Anti-ACADM antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX13677

GTX13677 IHC-P Image

IHC-P analysis of human liver using GTX13677 ACADM antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399