GeneTex
United States (US)

ACADM antibody

GTX100032_39408_IFA_19011506_205.jpg
GTX100032_IHC_19011506_670.jpg
GTX100032_WB_19011506_768.jpg

Cat No. GTX100032

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

HeLaS3

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ACADM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ACAD1 antibody, FLJ18227 antibody, FLJ93013 antibody, FLJ99884 antibody, MCAD antibody, MCADH antibody, ACADM antibody, "acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain antibody", "medium-chain specific acyl-CoA dehydrogenase, mitochondrial antibody", "acyl-CoA dehydrogenase, C-4 to C-12 straight chain antibody"

Cellular Localization

Mitochondrion matrix

Background

This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100032_39408_IFA_19011506_205.jpg

GTX100032 ICC/IF Image

ACADM antibody detects ACADM protein at mitochondria by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: ACADM protein stained by ACADM antibody (GTX100032) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX100032_IHC_19011506_670.jpg

GTX100032 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using ACADM(GTX100032) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100032_WB_19011506_768.jpg

GTX100032 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
10% SDS PAGE
GTX100032 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169