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ACADM antibody

Anti-ACADM antibody used in Western Blot (WB). GTX100487
Anti-ACADM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100487
Anti-ACADM antibody used in IHC (Paraffin sections) (IHC-P). GTX100487
Anti-ACADM antibody used in IHC (Paraffin sections) (IHC-P). GTX100487

Cat No. GTX100487

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Raji

Predict Reactivity

Bovine, Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ACADM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

acyl-CoA dehydrogenase medium chain,ACAD1,MCAD,MCADH

Cellular Localization

Mitochondrion matrix

Background

This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

  • Gene ID: 34 ACADM
  • UniProt: P11310 ACADM

Research Area

DATA IMAGES
Anti-ACADM antibody used in Western Blot (WB). GTX100487

GTX100487 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX100487 diluted at 1:1000

Anti-ACADM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100487

GTX100487 ICC/IF Image

ACADM antibody detects ACADM protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ACADM protein stained by ACADM antibody (GTX100487) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-ACADM antibody used in IHC (Paraffin sections) (IHC-P). GTX100487

GTX100487 IHC-P Image

ACADM antibody detects ACADM protein at mitochondria in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ACADM antibody (GTX100487) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACADM antibody used in IHC (Paraffin sections) (IHC-P). GTX100487

GTX100487 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using ACADM(GTX100487) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169