GeneTex
United States (US)

ACADM antibody

GTX100487_WB_19011506_158.jpg
GTX100487_40051_20170913_IFA_19011506_563.jpg
GTX100487_40051_20150415_IHC_M_19011506_927.jpg
GTX100487_40051_IHC_19011506_193.jpg

Cat No. GTX100487

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Raji

Predict Reactivity

Bovine, Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ACADM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ACAD1 antibody, FLJ18227 antibody, FLJ93013 antibody, FLJ99884 antibody, MCAD antibody, MCADH antibody, ACADM antibody, "acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain antibody", "medium-chain specific acyl-CoA dehydrogenase, mitochondrial antibody", "acyl-CoA dehydrogenase, C-4 to C-12 straight chain antibody"

Cellular Localization

Mitochondrion matrix

Background

This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100487_WB_19011506_158.jpg

GTX100487 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX100487 diluted at 1:1000

GTX100487_40051_20170913_IFA_19011506_563.jpg

GTX100487 ICC/IF Image

ACADM antibody detects ACADM protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ACADM protein stained by ACADM antibody (GTX100487) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX100487_40051_20150415_IHC_M_19011506_927.jpg

GTX100487 IHC-P Image

ACADM antibody detects ACADM protein at mitochondria in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ACADM antibody (GTX100487) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100487_40051_IHC_19011506_193.jpg

GTX100487 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using ACADM(GTX100487) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169