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ACADM antibody

Anti-ACADM antibody used in Western Blot (WB). GTX100488
Anti-ACADM antibody used in Western Blot (WB). GTX100488
Anti-ACADM antibody used in IHC (Paraffin sections) (IHC-P). GTX100488
Anti-ACADM antibody used in Western Blot (WB). GTX100488
Anti-ACADM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100488

Cat No. GTX100488

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

Molt-4 , Neuro2A , GL261 , C8D30 , PC-12

Predict Reactivity

Zebrafish, Bovine, Pig, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACADM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

acyl-CoA dehydrogenase medium chain , ACAD1 , MCAD , MCADH

Cellular Localization

Mitochondrion matrix

Background

This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

  • Gene ID: 34 ACADM
  • UniProt: P11310 ACADM

Research Area

DATA IMAGES

Anti-ACADM antibody used in Western Blot (WB). GTX100488

GTX100488 WB Image

ACADM antibody detects ACADM protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
10 % SDS-PAGE
ACADM antibody (GTX100488) dilution: 1:1000

Anti-ACADM antibody used in Western Blot (WB). GTX100488

GTX100488 WB Image

ACADM antibody detects ACADM protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
10 % SDS-PAGE
ACADM antibody (GTX100488) dilution: 1:1000

Anti-ACADM antibody used in IHC (Paraffin sections) (IHC-P). GTX100488

GTX100488 IHC-P Image

ACADM antibody detects ACADM protein at mitochondria on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
ACADM antibody (GTX100488) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ACADM antibody used in Western Blot (WB). GTX100488

GTX100488 WB Image

Sample(30 μg of whole cell lysate)
A:MOLT4(GTX27912)
7.5% SDS PAGE
GTX100488 diluted at 1:2000

Anti-ACADM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100488

GTX100488 ICC/IF Image

ACADM antibody detects ACADM protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ACADM protein stained by ACADM antibody (GTX100488) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169