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ACADM antibody

Anti-ACADM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32421
Anti-ACADM antibody used in Western Blot (WB). GTX32421

Cat No. GTX32421

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat, Pig
Package
100 μl ($329)
See all ACADM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ACADM (NP_000007.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

acyl-CoA dehydrogenase medium chain , ACAD1 , MCAD , MCADH

Cellular Localization

Mitochondrion matrix

Background

This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

  • Gene ID: 34 ACADM
  • UniProt: P11310 ACADM

Research Area

DATA IMAGES

Anti-ACADM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32421

GTX32421 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX32421 ACADM antibody.
Blue : DAPI
Dilution : 1:100

Anti-ACADM antibody used in Western Blot (WB). GTX32421

GTX32421 WB Image

WB analysis of various sample lysates using GTX32421 ACADM antibody.
Loading : 25ug per lane
Dilution : 1:1000

REFERENCE

Application Reference

Gonzalez-Hurtado E et al. Mol Metab 2018; 7 : 45-56 Fatty acid oxidation is required for active and quiescent brown adipose tissue maintenance and thermogenic programing.
Gonzalez-Hurtado E et al. Am J Physiol Endocrinol Metab 2017; 312 (5) : E381-E393 Loss of macrophage fatty acid oxidation does not potentiate systemic metabolic dysfunction.
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329