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ACADSB antibody

Overexpression
Anti-ACADSB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114231
Anti-ACADSB antibody used in IHC (Paraffin sections) (IHC-P). GTX114231
Anti-ACADSB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114231
Anti-ACADSB antibody used in Western Blot (WB). GTX114231
Anti-ACADSB antibody used in Western Blot (WB). GTX114231

Cat. No. GTX114231

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ACADSB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:5000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

HepG2 , ACADSB-transfected 293T

Predict Reactivity

Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACADSB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170067

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acyl-CoA dehydrogenase short/branched chain , 2-MEBCAD , ACAD7 , SBCAD

Cellular Localization

Mitochondrion matrix

Background

Short/branched chain acyl-CoA dehydrogenase(ACADSB) is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. Substrate specificity is the primary characteristic used to define members of this gene family. The ACADSB gene product has the greatest activity towards the short branched chain acyl-CoA derivative, (S)-2-methylbutyryl-CoA, but also reacts significantly with other 2-methyl branched chain substrates and with short straight chain acyl-CoAs. The cDNA encodes for a mitochondrial precursor protein which is cleaved upon mitochondrial import and predicted to yield a mature peptide of approximately 43.7-KDa. [provided by RefSeq]

Database

  • Gene ID: 36 ACADSB
  • UniProt: P45954 ACADSB

Research Area

DATA IMAGES

Anti-ACADSB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114231

GTX114231 ICC/IF Image

SBCAD antibody detects SBCAD protein at mitochondria by immunofluorescent analysis.
Sample: Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: SBCAD protein stained by SBCAD antibody (GTX114231) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-ACADSB antibody used in IHC (Paraffin sections) (IHC-P). GTX114231

GTX114231 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using SBCAD(GTX114231) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ACADSB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114231

GTX114231 ICC/IF Image

SBCAD antibody detects SBCAD protein at mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: SBCAD protein stained by SBCAD antibody (GTX114231) diluted at 1:5000.
Blue: Hoechst 33342 staining.
Anti-ACADSB antibody used in Western Blot (WB). GTX114231

GTX114231 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ACADSB antibody (GTX114231) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ACADSB antibody used in Western Blot (WB). GTX114231

GTX114231 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
10% SDS PAGE
GTX114231 diluted at 1:10000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169