GeneTex
  • Country / Location Selection

United States (US)

ACADVL antibody [N1C1]

Anti-ACADVL antibody [N1C1] used in Western Blot (WB). GTX114232
Anti-ACADVL antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX114232
Anti-ACADVL antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX114232
Anti-ACADVL antibody [N1C1] used in Western Blot (WB). GTX114232
Anti-ACADVL antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX114232
Anti-ACADVL antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX114232
Anti-ACADVL antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114232
Anti-ACADVL antibody [N1C1] used in Western Blot (WB). GTX114232
Anti-ACADVL antibody [N1C1] used in Western Blot (WB). GTX114232

Cat No. GTX114232

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

H1299 , HeLa , HepG2 , mouse liver

Predict Reactivity

Zebrafish, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACADVL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620453

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

acyl-CoA dehydrogenase very long chain , ACAD6 , LCACD , VLCAD

Cellular Localization

Mitochondrion inner membrane

Background

The protein encoded by this gene is targeted to the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenase is specific to long-chain and very-long-chain fatty acids. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

  • Gene ID: 37 ACADVL
  • UniProt: P49748 ACADVL

Research Area

DATA IMAGES

Anti-ACADVL antibody [N1C1] used in Western Blot (WB). GTX114232

GTX114232 WB Image

Wild-type (WT) and ACADVL knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACADVL antibody [N1C1] (GTX114232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ACADVL antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX114232

GTX114232 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H358 xenograft, using ACADVL(GTX114232) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ACADVL antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX114232

GTX114232 IHC-P Image

ACADVL antibody [N1C1] detects ACADVL protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human A549 xenograft.
ACADVL antibody [N1C1] (GTX114232) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ACADVL antibody [N1C1] used in Western Blot (WB). GTX114232

GTX114232 WB Image

Sample (50 μg of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX114232 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ACADVL antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX114232

GTX114232 IHC-P Image

ACADVL antibody [N1C1] detects ACADVL protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ACADVL stained by ACADVL antibody [N1C1] (GTX114232) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACADVL antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX114232

GTX114232 IHC-P Image

ACADVL antibody [N1C1] detects ACADVL protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
ACADVL stained by ACADVL antibody [N1C1] (GTX114232) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACADVL antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114232

GTX114232 ICC/IF Image

ACADVL antibody [N1C1] detects ACADVL protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ACADVL stained by ACADVL antibody [N1C1] (GTX114232) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-ACADVL antibody [N1C1] used in Western Blot (WB). GTX114232

GTX114232 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACADVL antibody [N1C1] (GTX114232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-ACADVL antibody [N1C1] used in Western Blot (WB). GTX114232

GTX114232 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACADVL antibody [N1C1] (GTX114232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

There are currently no reviews for ACADVL antibody [N1C1] (GTX114232). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf