GeneTex
United States (US)

ACAT1 (aa253-266) antibody, Internal

GTX88247_2449_WB_18122102_753.jpg

Cat No. GTX88247

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.01-0.03μg/ml
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88247-PEP).

Predict Reactivity

Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DEEYKRVDFSKVPK, from the internal region of the protein sequence according to NP_000010.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Acetyl-Coa Acetyltransferase 1,Acat,Mat,T2,Thil,Acat1

Background

This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]

Database

Research Area

DATA IMAGES
GTX88247_2449_WB_18122102_753.jpg

GTX88247 WB Image

WB analysis of mouse liver lysate using GTX88247 ACAT1 (aa253-266) antibody, Internal.
Dilution : 0.01µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329