GeneTex
United States (US)

ACAT1 (aa257-269) antibody, Internal

GTX88076_2447_WB_18121411_720.jpg
GTX88076_2448_WB_18121411_954.jpg

Cat No. GTX88076

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.01-0.03μg/ml
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88076-PEP).

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence C-KRVDFSKVPKLKT, from the internal region of the protein sequence according to NP_000010.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ACAT Antibody , ACAT1 Antibody , THIL Antibody , MAT Antibody , T2 Antibody

Background

This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]
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Database

Research Area

DATA IMAGES
GTX88076_2447_WB_18121411_720.jpg

GTX88076 WB Image

WB analysis of human liver lysate using GTX88076 ACAT1 (aa257-269) antibody, Internal.
Dilution : 0.01µg/ml
Loading : 35µg protein in RIPA buffer

GTX88076_2448_WB_18121411_954.jpg

GTX88076 WB Image

WB analysis of mouse (A) and rat (B) kidney lysates, and mouse (C) and rat (D) liver lysates using GTX88076 ACAT1 (aa257-269) antibody, Internal.
Dilution : 0.01µg/ml
Loading : 35µg protein in RIPA buffer