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ACAT1 antibody [AT15E5]

Anti-ACAT1 antibody [AT15E5] used in Western Blot (WB). GTX53681

Cat. No. GTX53681

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT15E5

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all ACAT1 products

APPLICATION

Application Note

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human ACAT1 (34-427aa) purified from E. coli

Purification

By protein-A affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acetyl-CoA acetyltransferase 1 , ACAT , MAT , T2 , THIL

Cellular Localization

Mitochondrion

Background

This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]

Database

  • Gene ID: 38 ACAT1
  • UniProt: P24752 ACAT1

Research Area

DATA IMAGES

Anti-ACAT1 antibody [AT15E5] used in Western Blot (WB). GTX53681

GTX53681 WB Image

WB analysis of indicated lysates (40ug per lane) using ACAT1 antibody at a dilution of 1:1,000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399