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ACAT1 antibody [N1N3]

Anti-ACAT1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102637
Anti-ACAT1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX102637
Anti-ACAT1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102637
Anti-ACAT1 antibody [N1N3] used in Western Blot (WB). GTX102637
Anti-ACAT1 antibody [N1N3] used in Western Blot (WB). GTX102637

Cat No. GTX102637

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

293T , A431 , Molt-4 , mouse brain , *F31 , *F90

Predict Reactivity

Rat, Zebrafish, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ACAT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Acetyl-Coa Acetyltransferase 1,Acat,Mat,T2,Thil,Acat1

Cellular Localization

Mitochondrion

Background

This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-ACAT1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102637

GTX102637 ICC/IF Image

ACAT1 antibody [N1N3] detects ACAT1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ACAT1 protein stained by ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

Anti-ACAT1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX102637

GTX102637 IHC-P Image

ACAT1 antibody [N1N3] detects ACAT1 protein at cytoplasm on human normal kidney by immunohistochemical analysis.
Sample: Paraffin-embedded human normal kidney.
ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ACAT1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102637

GTX102637 ICC/IF Image

ACAT1 antibody [N1N3] detects ACAT1 protein at mitochondria by immunofluorescent analysis.
Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min.
Green: ACAT1 protein stained by ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-ACAT1 antibody [N1N3] used in Western Blot (WB). GTX102637

GTX102637 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX102637 diluted at 1:1000

Anti-ACAT1 antibody [N1N3] used in Western Blot (WB). GTX102637

GTX102637 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:A431(GTX27909)
C:MOLT4(GTX27912)
10% SDS PAGE
GTX102637 diluted at 1:500

REFERENCE
REVIEW

ACAT1 antibody [N1N3] Cat No. GTX102637

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 01-Oct-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : F31 cells and F90 cells
Amount used : 30μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169