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United States (US)

ACAT1 antibody [N1N3]

GTX102637_39764_20150410_IFA_19011506_103.jpg
GTX102637_39764_20141128_IHC_19011506_821.jpg
GTX102637_39764_IFA_19011506_389.jpg
GTX102637_39764_WB_M_brain_19011506_488.jpg
GTX102637_WB_19011506_469.jpg

Cat No. GTX102637

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

293T , A431 , Molt-4 , mouse brain , *F31 , *F90

Predict Reactivity

Rat, Zebrafish, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ACAT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ACAT antibody, MAT antibody, T2 antibody, THIL antibody, ACAT1 antibody, mitochondrial acetoacetyl-CoA thiolase antibody, acetoacetyl-CoA thiolase antibody, acetyl-Coenzyme A acetyltransferase 1 antibody, "acetyl-CoA acetyltransferase, mitochondrial antibody", acetoacetyl Coenzyme A thiolase antibody, acetyl-CoA acetyltransferase 1 antibody

Cellular Localization

Mitochondrion

Background

This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102637_39764_20150410_IFA_19011506_103.jpg

GTX102637 ICC/IF Image

ACAT1 antibody [N1N3] detects ACAT1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ACAT1 protein stained by ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

GTX102637_39764_20141128_IHC_19011506_821.jpg

GTX102637 IHC-P Image

ACAT1 antibody [N1N3] detects ACAT1 protein at cytoplasm on human normal kidney by immunohistochemical analysis.
Sample: Paraffin-embedded human normal kidney.
ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102637_39764_IFA_19011506_389.jpg

GTX102637 ICC/IF Image

ACAT1 antibody [N1N3] detects ACAT1 protein at mitochondria by immunofluorescent analysis.
Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min.
Green: ACAT1 protein stained by ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX102637_39764_WB_M_brain_19011506_488.jpg

GTX102637 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX102637 diluted at 1:1000

GTX102637_WB_19011506_469.jpg

GTX102637 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:A431(GTX27909)
C:MOLT4(GTX27912)
10% SDS PAGE
GTX102637 diluted at 1:500

REFERENCE
REVIEW

ACAT1 antibody [N1N3] Cat No. GTX102637

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 01-Oct-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : F31 cells and F90 cells
Amount used : 30μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169