GeneTex
United States (US)

ACAT1 antibody

Cat No. GTX30683

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human, Mouse, Hamster
See all ACAT1 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:400
ICC/IF Assay dependent
IP 2 - 10 μg/ml lysate
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Specificity/Sensitivity

This product is specific for protein corresponding to ACAT1 - Sterol O-acyltransferase 1 (SOAT1) Uniprot# P35610, Gene ID# 6646. [Note:- ACAT1 is a symbol for both acyl-Coenzyme A: cholesterol acyltransferase 1 (SOAT1/ACAT1) and for acetyl-CoA acetyltransferase 1 (ACAT1/ THIL, Gene ID 38, Uniprot P24752) which often cause confusion].
PROPERTIES

Form

Liquid

Buffer

Serum, 0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A fusion protein construct that contains the N-terminal region of human ACAT1. [UniProt# P35610]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Acetyl-Coa Acetyltransferase 1,Acat,Mat,T2,Thil,Acat1

Cellular Localization

Mitochondrion

Background

This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq]

Database

Research Area

Package List Price ($)
$ 319