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ACAT1 antibody

Anti-ACAT1 antibody used in Western Blot (WB). GTX32987
Anti-ACAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32987
Anti-ACAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32987
Anti-ACAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32987
Anti-ACAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32987
Anti-ACAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32987
Anti-ACAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32987

Cat No. GTX32987

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human ACAT1 (NP_000010.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

acetyl-CoA acetyltransferase 1 , ACAT , MAT , T2 , THIL

Cellular Localization

Mitochondrion

Background

This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]

Database

  • Gene ID: 38 ACAT1
  • UniProt: P24752 ACAT1

Research Area

DATA IMAGES

Anti-ACAT1 antibody used in Western Blot (WB). GTX32987

GTX32987 WB Image

WB analysis of various sample lysates using GTX32987 ACAT1 antibody.
Loading : 25ug per lane
Dilution : 1:5000

Anti-ACAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32987

GTX32987 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX32987 ACAT1 antibody.
Dilution : 1:100

Anti-ACAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32987

GTX32987 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32987 ACAT1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-ACAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32987

GTX32987 ICC/IF Image

ICC/IF analysis of C6 cells using GTX32987 ACAT1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-ACAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32987

GTX32987 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX32987 ACAT1 antibody.
Dilution : 1:100

Anti-ACAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32987

GTX32987 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX32987 ACAT1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-ACAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32987

GTX32987 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX32987 ACAT1 antibody.
Dilution : 1:100

REFERENCE

There are currently no references for ACAT1 antibody (GTX32987). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329