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ACAT2 antibody [1B7]

Anti-ACAT2 antibody [1B7] used in Flow cytometry (FACS). GTX84968
Anti-ACAT2 antibody [1B7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84968
Anti-ACAT2 antibody [1B7] used in Western Blot (WB). GTX84968
Anti-ACAT2 antibody [1B7] used in IHC (Paraffin sections) (IHC-P). GTX84968
Anti-ACAT2 antibody [1B7] used in Western Blot (WB). GTX84968
Anti-ACAT2 antibody [1B7] used in Flow cytometry (FACS). GTX84968
Anti-ACAT2 antibody [1B7] used in IHC (Paraffin sections) (IHC-P). GTX84968
Anti-ACAT2 antibody [1B7] used in IHC (Paraffin sections) (IHC-P). GTX84968
Anti-ACAT2 antibody [1B7] used in IHC (Paraffin sections) (IHC-P). GTX84968
Anti-ACAT2 antibody [1B7] used in IHC (Paraffin sections) (IHC-P). GTX84968
Anti-ACAT2 antibody [1B7] used in IHC (Paraffin sections) (IHC-P). GTX84968
Anti-ACAT2 antibody [1B7] used in Western Blot (WB). GTX84968

Cat No. GTX84968

Host Mouse
Clonality Monoclonal
Clone Name 1B7
Isotype IgG2b
Application WB, ICC/IF, IHC-P, FACS
Reactivity Human, Rat, Dog, Monkey
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

41 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human ACAT2 (NP_0058826) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Acetyl-Coa Acetyltransferase 2,Acat2

Cellular Localization

Cytoplasm

Background

The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-ACAT2 antibody [1B7] used in Flow cytometry (FACS). GTX84968

GTX84968 FACS Image

FACS analysis of HEK293T cells transfected with either ACAT2 plasmid(Red) or empty vector control plasmid(Blue) using GTX84968 ACAT2 antibody [1B7].

Anti-ACAT2 antibody [1B7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84968

GTX84968 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with ACAT2 plasmid using GTX84968 ACAT2 antibody [1B7].

Anti-ACAT2 antibody [1B7] used in Western Blot (WB). GTX84968

GTX84968 WB Image

WB analysis of HEK293T cells transfected with ACAT2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84968 ACAT2 antibody [1B7].
Loading : 5 ug per lane

Anti-ACAT2 antibody [1B7] used in IHC (Paraffin sections) (IHC-P). GTX84968

GTX84968 IHC-P Image

IHC-P analysis of human liver tissue using GTX84968 ACAT2 antibody [1B7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

Anti-ACAT2 antibody [1B7] used in Western Blot (WB). GTX84968

GTX84968 WB Image

WB analysis of various cell lines using GTX84968 ACAT2 antibody [1B7].
Loading : 35 ug per lane

Anti-ACAT2 antibody [1B7] used in Flow cytometry (FACS). GTX84968

GTX84968 FACS Image

FACS analysis of Jurkat cells using GTX84968 ACAT2 antibody [1B7].
Red : Primary antibody
Blue : Negative control antibody

Anti-ACAT2 antibody [1B7] used in IHC (Paraffin sections) (IHC-P). GTX84968

GTX84968 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX84968 ACAT2 antibody [1B7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

Anti-ACAT2 antibody [1B7] used in IHC (Paraffin sections) (IHC-P). GTX84968

GTX84968 IHC-P Image

IHC-P analysis of human prostate tissue using GTX84968 ACAT2 antibody [1B7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

Anti-ACAT2 antibody [1B7] used in IHC (Paraffin sections) (IHC-P). GTX84968

GTX84968 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84968 ACAT2 antibody [1B7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

Anti-ACAT2 antibody [1B7] used in IHC (Paraffin sections) (IHC-P). GTX84968

GTX84968 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84968 ACAT2 antibody [1B7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

Anti-ACAT2 antibody [1B7] used in IHC (Paraffin sections) (IHC-P). GTX84968

GTX84968 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84968 ACAT2 antibody [1B7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

Anti-ACAT2 antibody [1B7] used in Western Blot (WB). GTX84968

GTX84968 WB Image

WB analysis of wild type and ACAT2 knock out HeLa cell lysate(10μg per lane) using GTX84968 ACAT2 antibody [1B7].
Dilution : 1:1000

Package List Price ($)
$ 379