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ACE antibody

Anti-ACE antibody used in IHC (Paraffin sections) (IHC-P). GTX130534
Anti-ACE antibody used in Western Blot (WB). GTX130534
Anti-ACE antibody used in IHC (Paraffin sections) (IHC-P). GTX130534
Anti-ACE antibody used in Western Blot (WB). GTX130534
Anti-ACE antibody used in Western Blot (WB). GTX130534
Anti-ACE antibody used in Western Blot (WB). GTX130534

Cat. No. GTX130534

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ACE products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

150 kDa. ( Note )

Positive Control

mouse lung

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human ACE. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886299

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

angiotensin I converting enzyme , ACE1 , CD143 , DCP , DCP1

Cellular Localization

Angiotensin-converting enzyme , soluble form: Secreted , Cell membrane

Background

This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Two most abundant alternatively spliced variants of this gene encode two isozymes - the somatic form and the testicular form that are equally active. Multiple additional alternatively spliced variants have been identified but their full length nature has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ACE antibody used in IHC (Paraffin sections) (IHC-P). GTX130534

GTX130534 IHC-P Image

ACE antibody detects ACE protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ACE stained by ACE antibody (GTX130534) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACE antibody used in Western Blot (WB). GTX130534

GTX130534 WB Image

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ACE antibody (GTX130534) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ACE antibody used in IHC (Paraffin sections) (IHC-P). GTX130534

GTX130534 IHC-P Image

ACE antibody detects ACE protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
ACE stained by ACE antibody (GTX130534) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACE antibody used in Western Blot (WB). GTX130534

GTX130534 WB Image

Human kidney (10 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ACE antibody (GTX130534) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ACE antibody used in Western Blot (WB). GTX130534

GTX130534 WB Image

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ACE antibody (GTX130534) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ACE antibody used in Western Blot (WB). GTX130534

GTX130534 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ACE antibody (GTX130534) diluted at 1:2000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169