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ACE2 antibody [GT19410]

KO/KD Comparison
Anti-ACE2 antibody [GT19410] used in Western Blot (WB). GTX635897
Anti-ACE2 antibody [GT19410] used in IHC (Paraffin sections) (IHC-P). GTX635897
Anti-ACE2 antibody [GT19410] used in ELISA (ELISA). GTX635897
Anti-ACE2 antibody [GT19410] used in Western Blot (WB). GTX635897
Anti-ACE2 antibody [GT19410] used in IHC (Paraffin sections) (IHC-P). GTX635897

Cat No. GTX635897

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT19410

Isotype

 IgG2b

Application

 WB, IHC-P, ELISA, Sandwich ELISA

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all ACE2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P 1:100-1:1000
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX635897 , Detection : GTX628914-01
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACE2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

angiotensin I converting enzyme 2 , ACEH

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ACE2 antibody [GT19410] used in Western Blot (WB). GTX635897

GTX635897 WB Image

KO/KD

Non-transfected (–) and transfected (+) Caco-2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [GT19410] (GTX635897) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ACE2 antibody [GT19410] used in IHC (Paraffin sections) (IHC-P). GTX635897

GTX635897 IHC-P Image

ACE2 antibody [GT19410] detects ACE2 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
ACE2 stained by ACE2 antibody [GT19410] (GTX635897) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACE2 antibody [GT19410] used in ELISA (ELISA). GTX635897

GTX635897 ELISA Image

Sandwich ELISA detection of recombinant Human ACE2 (ECD) protein, His and Avi tag (active) (GTX01550-pro) using ACE2 antibody [GT19410] (GTX635897) as capture antibody at concentration of 5 μg/mL and 6X His tag antibody [GT359] (HRP) (GTX628914-01) as detection antibody at concentration of 0.1 μg/mL.

Anti-ACE2 antibody [GT19410] used in Western Blot (WB). GTX635897

GTX635897 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [GT19410] (GTX635897) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ACE2 antibody [GT19410] used in IHC (Paraffin sections) (IHC-P). GTX635897

GTX635897 IHC-P Image

Comparison

ACE2 antibody [GT19410] detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
ACE2 stained by ACE2 antibody [GT19410] (GTX635897) diluted at 1:1000 and competitor's antibody diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min (left) and Tris-EDTA buffer, pH 9.0, 15min (right)

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

REFERENCE

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REVIEW

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Package List Price ($)
$ 329
$ 169