ACE2 antibody [GT19410]

Name: ACE2 antibody [GT19410]
Brand: GeneTex
SKU: GTX635897
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-ACE2 antibody [GT19410] used in Western Blot (WB). GTX635897

Anti-ACE2 antibody [GT19410] used in Lateral Flow (Lateral Flow). GTX635897

Anti-ACE2 antibody [GT19410] used in IHC (Paraffin sections) (IHC-P). GTX635897

Anti-ACE2 antibody [GT19410] used in ELISA (ELISA). GTX635897

Anti-ACE2 antibody [GT19410] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635897

Cat. No. GTX635897

Host	Mouse
Clonality	Monoclonal
Clone Name	GT19410
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, ELISA, Lateral Flow, Sandwich ELISA
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all ACE2 products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IHC-P	1:100-1:1000
ELISA	Assay dependent
Lateral Flow	Assay dependent
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture : GTX635897 , Detection : GTX628914-01

Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Cat, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACE2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A

Conjugation

Unconjugated

RRID

AB_2909920

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

angiotensin I converting enzyme 2 , ACEH

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]

Database

Gene ID: 59272 ACE2
UniProt: Q9BYF1 ACE2

Research Area

DATA IMAGES

GTX635897 WB Image

Non-transfected (–) and transfected (+) Caco-2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [GT19410] (GTX635897) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX635897 Lateral Flow Image

SARS-CoV-2 neutralizing antibody interferes with the binding of ACE2 and spike RBD proteins. The signal intensity of the test line (T) decreases due to competition by the neutralizing antibody, while the control line (C) demonstrates a valid test.

Neutralizing Antibody: Spike RBD antibody [HL1002] (GTX635791)
C Line:
Detector: Colloidal gold-conjugated human ACE2 (ECO) protein (GTX135683-pro) / Capture: Anti-human ACE2 antibody (GTX635897)
T Line:
Detector: Colloidal gold-conjugated human ACE2 (ECO) protein (GTX135683-pro) / Capture: SARS-CoV-2 Spike RBD protein

GTX635897 IHC-P Image

ACE2 antibody [GT19410] detects ACE2 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
ACE2 stained by ACE2 antibody [GT19410] (GTX635897) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX635897 ELISA Image

Sandwich ELISA detection of recombinant Human ACE2 (ECD) protein, His and Avi tag (active) (GTX01550-pro) using ACE2 antibody [GT19410] (GTX635897) as capture antibody at concentration of 5 μg/mL and 6X His tag antibody [GT359] (HRP) (GTX628914-01) as detection antibody at concentration of 0.1 μg/mL.

GTX635897 IHC-P Image

ACE2 antibody [GT19410] detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
ACE2 stained by ACE2 antibody [GT19410] (GTX635897) diluted at 1:1000 and competitor's antibody diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min (left) and Tris-EDTA buffer, pH 9.0, 15min (right)

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

GTX635897 ICC/IF Image

ACE2 antibody [GT19410] detects ACE2 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: Caco-2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ACE2 stained by ACE2 antibody [GT19410] (GTX635897) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX635897 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [GT19410] (GTX635897) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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REVIEW