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ACE2 antibody [GT19410]

Anti-ACE2 antibody [GT19410] used in Western Blot (WB). GTX635897
Anti-ACE2 antibody [GT19410] used in Lateral Flow (Lateral Flow). GTX635897
Anti-ACE2 antibody [GT19410] used in Western Blot (WB). GTX635897
Anti-ACE2 antibody [GT19410] used in IHC (Paraffin sections) (IHC-P). GTX635897
Anti-ACE2 antibody [GT19410] used in ELISA (ELISA). GTX635897
Anti-ACE2 antibody [GT19410] used in IHC (Paraffin sections) (IHC-P). GTX635897

Cat No. GTX635897

Host

Mouse

Clonality

Monoclonal

Clone Name

GT19410

Isotype

IgG2b

Application

WB, IHC-P, ELISA, Lateral Flow, Sandwich ELISA

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P 1:100-1:1000
ELISA Assay dependent
Lateral Flow Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX635897 , Detection : GTX628914-01

Not tested in other applications.

Calculated MW

92 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACE2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

angiotensin I converting enzyme 2 , ACEH

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ACE2 antibody [GT19410] used in Western Blot (WB). GTX635897

GTX635897 WB Image

Non-transfected (–) and transfected (+) Caco-2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [GT19410] (GTX635897) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ACE2 antibody [GT19410] used in Lateral Flow (Lateral Flow). GTX635897

GTX635897 Lateral Flow Image

SARS-CoV-2 neutralizing antibody interferes with the binding of ACE2 and spike RBD proteins. The signal intensity of the test line (T) decreases due to competition by the neutralizing antibody, while the control line (C) demonstrates a valid test.

Neutralizing Antibody: Spike RBD antibody [HL1002] (GTX635791)
C Line:
Detector: Colloidal gold-conjugated human ACE2 (ECO) protein (GTX135683-pro) / Capture: Anti-human ACE2 antibody (GTX635897)
T Line:
Detector: Colloidal gold-conjugated human ACE2 (ECO) protein (GTX135683-pro) / Capture: SARS-CoV-2 Spike RBD protein

Anti-ACE2 antibody [GT19410] used in Western Blot (WB). GTX635897

GTX635897 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [GT19410] (GTX635897) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ACE2 antibody [GT19410] used in IHC (Paraffin sections) (IHC-P). GTX635897

GTX635897 IHC-P Image

ACE2 antibody [GT19410] detects ACE2 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
ACE2 stained by ACE2 antibody [GT19410] (GTX635897) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACE2 antibody [GT19410] used in ELISA (ELISA). GTX635897

GTX635897 ELISA Image

Sandwich ELISA detection of recombinant Human ACE2 (ECD) protein, His and Avi tag (active) (GTX01550-pro) using ACE2 antibody [GT19410] (GTX635897) as capture antibody at concentration of 5 μg/mL and 6X His tag antibody [GT359] (HRP) (GTX628914-01) as detection antibody at concentration of 0.1 μg/mL.

Anti-ACE2 antibody [GT19410] used in IHC (Paraffin sections) (IHC-P). GTX635897

GTX635897 IHC-P Image

ACE2 antibody [GT19410] detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
ACE2 stained by ACE2 antibody [GT19410] (GTX635897) diluted at 1:1000 and competitor's antibody diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min (left) and Tris-EDTA buffer, pH 9.0, 15min (right)

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

REFERENCE

There are currently no references for ACE2 antibody [GT19410] (GTX635897). Be the first to share your publications with this product.

REVIEW

ACE2 antibody [GT19410] Cat No. GTX635897

Rating ( Average 5 based on 1 users reviews)
Application
Lateral Flow(Lateral Flow) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Nov-2021
Score :
Application Tested : Lateral Flow
Figure Note : SARS-CoV-2 neutralizing antibody interferes with the binding of ACE2 and spike RBD proteins.
Package List Price ($)
$ 329
$ 169
Custom Conjugations