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ACE2 antibody [HL1092]

Recombinant antibody KO/KD Comparison Overexpression
Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265
Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265
Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265
Anti-ACE2 antibody [HL1092] used in IHC (Paraffin sections) (IHC-P). GTX636265
Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265
Anti-ACE2 antibody [HL1092] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636265

Cat. No. GTX636265

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1092

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, African green monkey
Package
100 μl ($399),
25 μl ($169)
See all ACE2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Product Note

We do not recommend use of this product for Mouse samples.

Predict Reactivity

Cat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ACE2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909957

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

angiotensin I converting enzyme 2 , ACEH

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265

GTX636265 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265

GTX636265 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265

GTX636265 WB Image

Various whole cell and tissue extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-ACE2 antibody [HL1092] used in IHC (Paraffin sections) (IHC-P). GTX636265

GTX636265 IHC-P Image

Comparison

ACE2 antibody [HL1092] detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
ACE2 stained by ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000 and competitor's antibody diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min (left) and Tris-EDTA buffer, pH 9.0, 15min (right)

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265

GTX636265 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ACE2 antibody [HL1092] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636265

GTX636265 ICC/IF Image

ACE2 antibody [HL1092] detects ACE2 protein at cell membrane by immunofluorescent analysis.
Sample: VeroE6 cells were fixed in ice-cold MeOH for 5 min.
Green: ACE2 stained by ACE2 antibody [HL1092] (GTX636265) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations