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ACE2 antibody [HL1092]

Recombinant antibody KO/KD Comparison Overexpression
Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265
Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265
Anti-ACE2 antibody [HL1092] used in IHC (Paraffin sections) (IHC-P). GTX636265
Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265
Anti-ACE2 antibody [HL1092] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636265
Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265

Cat No. GTX636265

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1092

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, African green monkey
Package
100 μl ($379),
25 μl ($169)
See all ACE2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Product Note

We do not recommend use of this product for Mouse samples.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ACE2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909957

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

angiotensin I converting enzyme 2 , ACEH

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265

GTX636265 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265

GTX636265 WB Image

Various whole cell and tissue extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-ACE2 antibody [HL1092] used in IHC (Paraffin sections) (IHC-P). GTX636265

GTX636265 IHC-P Image

Comparison

ACE2 antibody [HL1092] detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
ACE2 stained by ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000 and competitor's antibody diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min (left) and Tris-EDTA buffer, pH 9.0, 15min (right)

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265

GTX636265 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ACE2 antibody [HL1092] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636265

GTX636265 ICC/IF Image

ACE2 antibody [HL1092] detects ACE2 protein at cell membrane by immunofluorescent analysis.
Sample: VeroE6 cells were fixed in ice-cold MeOH for 5 min.
Green: ACE2 stained by ACE2 antibody [HL1092] (GTX636265) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265

GTX636265 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 379
$ 169
Custom Conjugations