GeneTex
  • Country / Location Selection

United States (US)

ACE2 antibody [N1N2], N-term

Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in ELISA (ELISA). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in ELISA (ELISA). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395

Cat. No. GTX101395

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

Human, Mouse, Rat, African green monkey
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
FACS Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Predict Reactivity

Cat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human ACE2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240451

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

angiotensin I converting enzyme 2 , ACEH

Cellular Localization

Processed angiotensin-converting enzyme 2: Secreted , Cell membrane

Background

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395

GTX101395 WB Image

Non-transfected (–) and transfected (+) Caco-2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395

GTX101395 WB Image

Untreated (–) and treated (+) Vero E6 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ACE2 antibody [N1N2], N-term used in ELISA (ELISA). GTX101395

GTX101395 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag protein (GTX135683-pro) (15.63-1000 ng/mL). Coated protein probed with ACE2 antibody [N1N2], N-term (GTX101395) (0.1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395

GTX101395 WB Image

GTX135683-pro Human ACE2 (ECD) protein , mouse IgG Fc tag protein (1 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395

GTX101395 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ACE2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101395

GTX101395 ICC/IF Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane by immunofluorescent analysis.
Sample: Vero E6 cells were fixed in ice-cold MeOH for 5 min.
Green: ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ACE2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101395

GTX101395 ICC/IF Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: Caco-2 cells were fixed in ice-cold MeOH for 5 min.
Green: ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395

GTX101395 WB Image

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ACE2 antibody [N1N2], N-term used in ELISA (ELISA). GTX101395

GTX101395 ELISA Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (4.5-10000 ng/mL). Bound protein was detected by ACE2 antibody [N1N2], N-term (GTX101395) (0.5 μg/mL) and Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395

GTX101395 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ACE2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101395

GTX101395 ICC/IF Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane by immunofluorescent analysis.
Sample: Vero E6 cells were fixed in ice-cold MeOH for 5 min.
Green: ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

ACE2 antibody [N1N2], N-term Cat. No. GTX101395

Rating ( Average 5 based on 8 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 2 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Flow cytometry(FACS) ( Average 5 based on 5 users reviews)
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2022 in Biomedicines. PMID: 35625919
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2022 in Biomedicines. PMID: 35625919
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : FACS
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2022 in Biomedicines. PMID: 35625919
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2022 in Biomedicines. PMID: 35625919
Date : Anonymous submitted on 30-Jun-2022
Score :
Application Tested : FACS
Sample Species : Hu
Sample : 293T cells with/without Human ACE2 transfection
Blocking : 1% FBS in PBS, 4°C, 0.25Hr
Primary Antibody : 1:250, 4°C, 0.5Hr
Date : Anonymous submitted on 13-Oct-2020
Score :
Application Tested : FACS
Sample Species : Hu
Sample : Human ACE2 transfected 293T cells
Primary Antibody : 1:100, 4°C, 0.5Hr
Figure Note : Alexa 647-anti-Rabbit-IgG was used for secondary antibody to detect ACE2 expression (shown in red). Normal rabbit IgG instead of anti-ACE2 antibody was used as negative control (shown in blue)
Date : Anonymous submitted on 22-Mar-2020
Score :
Application Tested : FACS
Sample Species : Hu
Sample : THP-1 cells
Blocking : 1% FBS in PBS, 4°C, 0.25Hr
Primary Antibody : 1:250, 4°C, 0.5Hr
Date : Anonymous submitted on 22-Mar-2020
Score :
Application Tested : FACS
Sample Species : Hu
Sample : K562 cells
Blocking : 1% FBS in PBS, 4°C, 0.25Hr
Primary Antibody : 1:250, 4°C, 0.5Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169