ACE2 antibody [N1N2], N-term

Name: ACE2 antibody [N1N2], N-term
Brand: GeneTex
SKU: GTX101395
Price: 399.00 USD
Rating: 5 (8 reviews)

Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395

Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395

Anti-ACE2 antibody [N1N2], N-term used in ELISA (ELISA). GTX101395

Anti-ACE2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101395

Cat. No. GTX101395

Reference 22

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity	Human, Mouse, Rat, African green monkey
Package	100 μl ($399), 25 μl ($169)

See all ACE2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000
FACS	Assay dependent
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Predict Reactivity

Cat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ACE2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240451

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

angiotensin I converting enzyme 2 , ACEH

Cellular Localization

Processed angiotensin-converting enzyme 2: Secreted , Cell membrane

Background

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq]

Database

Gene ID: 59272 ACE2
UniProt: Q9BYF1 ACE2

Research Area

DATA IMAGES

GTX101395 WB Image

Non-transfected (–) and transfected (+) Caco-2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101395 WB Image

Untreated (–) and treated (+) Vero E6 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101395 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag protein (GTX135683-pro) (15.63-1000 ng/mL). Coated protein probed with ACE2 antibody [N1N2], N-term (GTX101395) (0.1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101395 WB Image

GTX135683-pro Human ACE2 (ECD) protein , mouse IgG Fc tag protein (1 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101395 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX101395 ICC/IF Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane by immunofluorescent analysis.
Sample: Vero E6 cells were fixed in ice-cold MeOH for 5 min.
Green: ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101395 ICC/IF Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: Caco-2 cells were fixed in ice-cold MeOH for 5 min.
Green: ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101395 WB Image

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101395 ELISA Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (4.5-10000 ng/mL). Bound protein was detected by ACE2 antibody [N1N2], N-term (GTX101395) (0.5 μg/mL) and Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101395 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101395 ICC/IF Image

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Isobe A et al. Commun Biol 2022; 5 (1) : 1188 ACE2 N-glycosylation modulates interactions with SARS-CoV-2 spike protein in a site-specific manner.

Chien LH et al. Biomed Pharmacother 2022; 153 : 113434 Study on the potential of Sanghuangporus sanghuang and its components as COVID-19 spike protein receptor binding domain inhibitors.

Application : WB

Reactivity : Human

Sun TK et al. Int J Mol Sci 2022; 23 (23) Schizophyllum commune Reduces Expression of the SARS-CoV-2 Receptors ACE2 and TMPRSS2.

Application : WB

Reactivity : Human

Wang WJ et al. J Food Biochem 2022; 46 (10) : e14354 Peimine inhibits variants of SARS-CoV-2 cell entry via blocking the interaction between viral spike protein and ACE2.

Zhenfeng Wang et al. Cell Mol Immunol 2022; 19 (2) : 210-221 SARS-CoV-2 treatment effects induced by ACE2-expressing microparticles are explained by the oxidized cholesterol-increased endosomal pH of alveolar macrophages.

Application : ICC/IF, FACS

Reactivity : Human

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REVIEW

ACE2 antibody [N1N2], N-term Cat. No. GTX101395

Rating

( Average 5 based on 8 users reviews)

Application

Immunocytochemistry/ Immunofluorescence(ICC/IF)		( Average 5 based on 2 users reviews)
IHC (Paraffin sections)(IHC-P)		( Average 5 based on 1 users reviews)
Flow cytometry(FACS)		( Average 5 based on 5 users reviews)

Date : Anonymous submitted on 03-Oct-2022

Score :

Application Tested : IHC-P

Sample Species : Hu

Figure Note : The data was peer reviewed and published in the 2022 in Biomedicines. PMID: 35625919

Date : Anonymous submitted on 03-Oct-2022

Score :

Application Tested : ICC/IF

Sample Species : Hu

Figure Note : The data was peer reviewed and published in the 2022 in Biomedicines. PMID: 35625919

Anti-ACE2 antibody [N1N2], N-term used in Flow cytometry (FACS). GTX101395

Date : Anonymous submitted on 03-Oct-2022

Score :

Application Tested : FACS

Sample Species : Hu

Figure Note : The data was peer reviewed and published in the 2022 in Biomedicines. PMID: 35625919

Date : Anonymous submitted on 03-Oct-2022

Score :

Application Tested : ICC/IF

Sample Species : Hu

Figure Note : The data was peer reviewed and published in the 2022 in Biomedicines. PMID: 35625919

Date : Anonymous submitted on 30-Jun-2022

Score :

Application Tested : FACS

Sample Species : Hu

Sample : 293T cells with/without Human ACE2 transfection

Blocking : 1% FBS in PBS, 4°C, 0.25Hr

Primary Antibody : 1:250, 4°C, 0.5Hr

Date : Anonymous submitted on 13-Oct-2020

Score :

Application Tested : FACS

Sample Species : Hu

Sample : Human ACE2 transfected 293T cells

Primary Antibody : 1:100, 4°C, 0.5Hr

Figure Note : Alexa 647-anti-Rabbit-IgG was used for secondary antibody to detect ACE2 expression (shown in red). Normal rabbit IgG instead of anti-ACE2 antibody was used as negative control (shown in blue)