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ACE2 antibody [N1N2], N-term

Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in ELISA (ELISA). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in ELISA (ELISA). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395

Cat No. GTX101395

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
FACS Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

A549 , HepG2

Predict Reactivity

Cat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ACE2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

angiotensin I converting enzyme 2 , ACEH

Cellular Localization

Processed angiotensin-converting enzyme 2: Secreted , Cell membrane

Background

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACE2 antibody [N1N2], N-term used in ELISA (ELISA). GTX101395

GTX101395 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag protein (GTX135683-pro) (15.63-1000 ng/mL). Coated protein probed with ACE2 antibody [N1N2], N-term (GTX101395) (0.1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395

GTX101395 WB Image

GTX135683-pro Human ACE2 (ECD) protein , mouse IgG Fc tag protein (1 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395

GTX101395 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication:
Reference:PMID: 16282461

Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395

GTX101395 WB Image

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at secreted on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
ACE2 antibody [N1N2], N-term (GTX101395) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395

GTX101395 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ACE2 antibody [N1N2], N-term used in ELISA (ELISA). GTX101395

GTX101395 ELISA Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (4.5-10000 ng/mL). Bound protein was detected by ACE2 antibody [N1N2], N-term (GTX101395) (0.5 μg/mL) and Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACE2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101395

GTX101395 ICC/IF Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane by immunofluorescent analysis.
Sample: Vero E6 cells were fixed in ice-cold MeOH for 5 min.
Green: ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395

GTX101395 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ACE2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101395

GTX101395 ICC/IF Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane by immunofluorescent analysis.
Sample: Vero E6 cells were fixed in ice-cold MeOH for 5 min.
Green: ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

ACE2 antibody [N1N2], N-term Cat No. GTX101395

Rating ( Average 5 based on 3 users reviews)
Application
Flow cytometry(FACS) ( Average 5 based on 3 users reviews)
Date : Anonymous submitted on 13-Oct-2020
Score :
Application Tested : FACS
Sample Species : Hu
Sample : Human ACE2 transfected 293T cells
Primary Antibody : 1:100, 4°C, 0.5Hr
Figure Note : Alexa 647-anti-Rabbit-IgG was used for secondary antibody to detect ACE2 expression (shown in red). Normal rabbit IgG instead of anti-ACE2 antibody was used as negative control (shown in blue)
Date : Anonymous submitted on 22-Mar-2020
Score :
Application Tested : FACS
Sample Species : Hu
Sample : THP-1 cells
Blocking : 1% FBS in PBS, 4°C, 0.25Hr
Primary Antibody : 1:250, 4°C, 0.5Hr
Date : Anonymous submitted on 22-Mar-2020
Score :
Application Tested : FACS
Sample Species : Hu
Sample : K562 cells
Blocking : 1% FBS in PBS, 4°C, 0.25Hr
Primary Antibody : 1:250, 4°C, 0.5Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf