ACE2 antibody [N1N2], N-term

Name: ACE2 antibody [N1N2], N-term
Brand: GeneTex
SKU: GTX101395
Price: 399.00 USD
Rating: 5 (4 reviews)

Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395

Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395

Anti-ACE2 antibody [N1N2], N-term used in ELISA (ELISA). GTX101395

Anti-ACE2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101395

Anti-ACE2 antibody [N1N2], N-term used in Flow cytometry (FACS). GTX101395

Cat. No. GTX101395

Reference 27

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity	Human, Mouse, Rat, African green monkey
Package	100 μl ($399), 25 μl ($169)

See all ACE2 products

View product citations for antibody GTX101395 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000
FACS	Assay dependent
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Predict Reactivity

Cat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human ACE2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240451

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

angiotensin I converting enzyme 2 , ACEH

Cellular Localization

Processed angiotensin-converting enzyme 2: Secreted , Cell membrane

Background

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq]

Database

Gene ID: 59272 ACE2
UniProt: Q9BYF1 ACE2

Research Area

DATA IMAGES

GTX101395 WB Image

Non-transfected (–) and transfected (+) Caco-2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101395 WB Image

Untreated (–) and treated (+) Vero E6 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101395 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag protein (GTX135683-pro) (15.63-1000 ng/mL). Coated protein probed with ACE2 antibody [N1N2], N-term (GTX101395) (0.1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101395 WB Image

GTX135683-pro Human ACE2 (ECD) protein , mouse IgG Fc tag protein (1 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101395 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX101395 ICC/IF Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane by immunofluorescent analysis.
Sample: Vero E6 cells were fixed in ice-cold MeOH for 5 min.
Green: ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101395 ICC/IF Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: Caco-2 cells were fixed in ice-cold MeOH for 5 min.
Green: ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101395 WB Image

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101395 ELISA Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (4.5-10000 ng/mL). Bound protein was detected by ACE2 antibody [N1N2], N-term (GTX101395) (0.5 μg/mL) and Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101395 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101395 ICC/IF Image

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101395 ICC/IF Image

The data was published in the 2022 in Biomedicines. PMID: 35625919

GTX101395 FACS Image

The data was published in the 2022 in Biomedicines. PMID: 35625919

GTX101395 IHC-P Image

The data was published in the 2022 in Biomedicines. PMID: 35625919

GTX101395 ICC/IF Image

The data was published in the 2022 in Biomedicines. PMID: 35625919

REFERENCE

Application Reference

Lin CH et al. Int J Mol Sci 2024; 25 (5) Inhibitory Efficacy of Main Components of Scutellaria baicalensis on the Interaction between Spike Protein of SARS-CoV-2 and Human Angiotensin-Converting Enzyme II.

A Escalera et al. Emerg Microbes Infect 2023; : 2297553 The impact of S2 mutations on Omicron SARS-CoV-2 cell surface expression and fusogenicity

Application : WB

Chen YR et al. Int J Mol Sci 2023; 24 (20) Anisomeles indica Extracts and Their Constituents Suppress the Protein Expression of ACE2 and TMPRSS2 In Vivo and In Vitro.

Resnick JD et al. bioRxiv 2023; Growth media affects susceptibility of air-lifted human nasal epithelial cell cultures to SARS-CoV2, but not Influenza A, virus infection.

Joh-Sin Wu et al. Viruses 2023; 15 (8) : 1762 Development of Zika Virus Mini-Replicon Based Single-Round Infectious Particles as Gene Delivery Vehicles

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REVIEW

ACE2 antibody [N1N2], N-term Cat. No. GTX101395

Rating

( Average 5 based on 4 users reviews)

Application

Flow cytometry(FACS)

( Average 5 based on 4 users reviews)

Date : Anonymous submitted on 30-Jun-2022

Score :

Application Tested : FACS

Sample Species : Hu

Sample : 293T cells with/without Human ACE2 transfection

Blocking : 1% FBS in PBS, 4°C, 0.25Hr

Primary Antibody : 1:250, 4°C, 0.5Hr

Date : Anonymous submitted on 13-Oct-2020

Score :

Application Tested : FACS

Sample Species : Hu

Sample : Human ACE2 transfected 293T cells

Primary Antibody : 1:100, 4°C, 0.5Hr

Figure Note : Alexa 647-anti-Rabbit-IgG was used for secondary antibody to detect ACE2 expression (shown in red). Normal rabbit IgG instead of anti-ACE2 antibody was used as negative control (shown in blue)