Non-transfected (–) and transfected (+) Caco-2 whole cell extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ACE2 antibody (GTX135403) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody (GTX135403) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.