APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5 - 2 μg/mL |
| Assay dependent |
| 2 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Kidney Tissue Lysate(GTX29995)
Product Note
Anti-ACE2 has no cross response to ACE1.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
ACE2 antibody was raised against a synthetic peptide corresponding to amino acids near the center of human ACE2.The immunogen is located within amino acids 150 - 200 of ACE2.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
angiotensin I converting enzyme 2 , ACEH
Cellular Localization
Cytoplasm
Background
Angiotensin converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently ACE2 has also been shown to be a functional receptor of the SARS coronavirus. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function. ACE1 inhibitors such as catopril and lisinopril do not block ACE2 activity. ACE2 is restricted to the testis, heart, kidney and colon. The full length sequence for ACE2 encodes an 805 amino acid protein with predicted mass of 92.46 kDa and a pI of 5.22. No cross reactivity to ACE1.
Database
Research Area
DATA IMAGES
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GTX15349 ICC/IF Image
IHC-P analysis of human kidney tissue using GTX15349 ACE2 antibody.
Working concentration : 2 μg/ml
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GTX15349 WB Image
WB analysis of human kidney lysate using GTX15349 ACE2 antibody.
Working concentration : 2 μg/ml
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GTX15349 IHC-P Image
IHC-P analysis of human kidney tissue using GTX15349 ACE2 antibody.
Working concentration : 2 μg/ml
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GTX15349 WB Image
WB analysis of various samples using GTX15349 ACE2 antibody.
Loading: 15 μg per lane
Dilution: 4μg/ml
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GTX15349 WB Image
WB analysis of mouse stomach tissue lysate using GTX15349 ACE2 antibody.
Loading: 15 μg per lane
Dilution: 4μg/ml
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GTX15349 ICC/IF Image
ICC/IF analysis of Caco2 cells using GTX15349 ACE2 antibody.
Dilution: 5μg/ml
Green: ACE2
Blue: DAPI
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GTX15349 WB Image
WB analysis of rat brain tissue lysate using GTX15349 ACE2 antibody.
Loading: 15 μg per lane
Dilution: 4μg/ml
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REFERENCE
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REVIEW
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