GeneTex
United States (US)

ACK1 antibody

ACK1-antibody_IP_GTX14776-1_18121409_653.jpg

Cat No. GTX14776

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Mouse, Rat
APPLICATION

Application Note

IP: Use at a dilution of 1/200. WB: Use at a dilution of 1/500. Detects a band of approximately 120 kDa. The antibody also reacts to a 100 kDa protein in various breast cancer cell lines. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

115 kDa. ( Note )

Positive Control

Tested with breast cancer cell lines.
PROPERTIES

Form

Liquid

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide – Immunogen sequence information is commercially sensitive

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tyrosine Kinase Non Receptor 2,Ack,Ack-1,Ack1,P21Cdc42Hs,Tnk2

Cellular Localization

Cell Membrane

Background

This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway.

Database

Research Area

DATA IMAGES
ACK1-antibody_IP_GTX14776-1_18121409_653.jpg

GTX14776 IP Image

IP with GTX14776 (1:500) using 250 µl of solubilized COS1 cells expressing constitutively active form of ACK1. Western blot with GTX14776 at 1:500. Apparent MW of ACK1 is 100 kDa.

Package List Price ($)
$ 329