GeneTex
United States (US)

ACOT4 antibody

ACOT4-antibody-GTX87881-WB-1_18122102_635.jpg
ACOT4-antibody-GTX87881-ICC_IF-1_18122102_162.jpg

Cat No. GTX87881

Host Rabbit
Clonality Polyclonal
Application WB, ICC/IF, ELISA
Reactivity Human
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
IF: 1:100~1:500
ELISA: 1:20000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

46 kDa. ( Note )

Specificity/Sensitivity

ACOT4 Antibody detects endogenous levels of total ACOT4 protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human ACOT4.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Acyl-Coa Thioesterase 4,Pte-Ib,Pte1B,Pte2B,Acot4

Background

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (By similarity). Succinyl-CoA thioesterase that also hydrolyzes long chain saturated and unsaturated monocarboxylic acyl-CoAs.

Database

DATA IMAGES
ACOT4-antibody-GTX87881-WB-1_18122102_635.jpg

GTX87881 WB Image

Western blot analysis of extracts from HeLa/COLO cells, using ACOT4 Antibody. The lane on the right is treated with the synthesized peptide.

ACOT4-antibody-GTX87881-ICC_IF-1_18122102_162.jpg

GTX87881 ICC/IF Image

Immunofluorescence analysis of MCF7 cells, using ACOT4 Antibody. The picture on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339