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ACOT8 antibody [AT4D10]

Anti-ACOT8 antibody [AT4D10] used in Flow cytometry (FACS). GTX57615
Anti-ACOT8 antibody [AT4D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57615
Anti-ACOT8 antibody [AT4D10] used in Western Blot (WB). GTX57615

Cat. No. GTX57615

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT4D10

Isotype

 IgG2a

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($399)
See all ACOT8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT4D10 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ACOT10 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acyl-CoA thioesterase 8 , ACOT8 , HNAACTE , NAP1 , PTE1 , PTE2 , acylCoA thioesterase 8 , hACTEIII , hTE

Cellular Localization

Cytoplasm,Peroxisome matrix

Background

The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. [provided by RefSeq, Oct 2010]

Database

Research Area

DATA IMAGES

Anti-ACOT8 antibody [AT4D10] used in Flow cytometry (FACS). GTX57615

GTX57615 FACS Image

FACS analysis of CTLL-2 cells using GTX57615 ACOT8 antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg

Anti-ACOT8 antibody [AT4D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57615

GTX57615 ICC/IF Image

ICC/IF analysis of A549 cells using GTX57615 ACOT8 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-ACOT8 antibody [AT4D10] used in Western Blot (WB). GTX57615

GTX57615 WB Image

WB analysis of various samples using GTX57615 ACOT8 antibody.
Lane 1 : Recombinant Human ACOT8 protein (10 ng)
Lane 2 : Ramos whole cell lysate (40 μg)
Lane 3 : A431 whole cell lysate (40 μg)
Lane 4 : 293T whole cell lysate (40 μg)
Lane 5 : A549 whole cell lysate (40 μg)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399