GeneTex
United States (US)

ACOT8 antibody [N1C3]

GTX103960_WB_19011506_727.jpg
GTX103960_39967_20141128_WB_R_liver_19011506_242.jpg
GTX103960_39967_20141128_WB_19011506_585.jpg
GTX103960_39967_20141128_WB_M_liver_19011506_383.jpg

Cat No. GTX103960

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , HepG2 , Molt-4 , Raji , mouse liver , rat liver

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACOT8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Acyl-Coa Thioesterase 8,Hnaacte,Nap1,Pte-1,Pte-2,Pte1,Pte2,Hacte-Iii,Hte,Acot8

Cellular Localization

Peroxisome

Background

The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103960_WB_19011506_727.jpg

GTX103960 WB Image

Sample(30 ug whole cell lysate)
A:MOLT4 (GTX27912)
B:Raji (GTX27908)
10% SDS PAGE
GTX103960 diluted at 1:1000

GTX103960_39967_20141128_WB_R_liver_19011506_242.jpg

GTX103960 WB Image

ACOT8 antibody detects ACOT8 protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with ACOT8 antibody (GTX103960) diluted by 1:1000.

GTX103960_39967_20141128_WB_19011506_585.jpg

GTX103960 WB Image

ACOT8 antibody detects ACOT8 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ACOT8 antibody (GTX103960) diluted by 1:1000.

GTX103960_39967_20141128_WB_M_liver_19011506_383.jpg

GTX103960 WB Image

ACOT8 antibody detects ACOT8 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with ACOT8 antibody (GTX103960) diluted by 1:1000.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169