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ACOT8 antibody [N1C3]

Anti-ACOT8 antibody [N1C3] used in Western Blot (WB). GTX103960
Anti-ACOT8 antibody [N1C3] used in Western Blot (WB). GTX103960
Anti-ACOT8 antibody [N1C3] used in Western Blot (WB). GTX103960
Anti-ACOT8 antibody [N1C3] used in Western Blot (WB). GTX103960

Cat. No. GTX103960

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ACOT8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

Molt-4 , Raji , 293T , HepG2 , mouse liver , rat liver

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACOT8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949568

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acyl-CoA thioesterase 8 , HNAACTE , NAP1 , PTE-1 , PTE-2 , PTE1 , PTE2 , hACTE-III , hTE

Cellular Localization

Peroxisome

Background

The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ACOT8 antibody [N1C3] used in Western Blot (WB). GTX103960

GTX103960 WB Image

ACOT8 antibody detects ACOT8 protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with ACOT8 antibody (GTX103960) diluted by 1:1000.

Anti-ACOT8 antibody [N1C3] used in Western Blot (WB). GTX103960

GTX103960 WB Image

ACOT8 antibody detects ACOT8 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with ACOT8 antibody (GTX103960) diluted by 1:1000.

Anti-ACOT8 antibody [N1C3] used in Western Blot (WB). GTX103960

GTX103960 WB Image

Sample(30 ug whole cell lysate)
A:MOLT4 (GTX27912)
B:Raji (GTX27908)
10% SDS PAGE
GTX103960 diluted at 1:1000

Anti-ACOT8 antibody [N1C3] used in Western Blot (WB). GTX103960

GTX103960 WB Image

ACOT8 antibody detects ACOT8 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ACOT8 antibody (GTX103960) diluted by 1:1000.

REFERENCE

Application Reference

Liu WL et al. Molecules 2019; 22 (6) : pii: E929 Curcumin Inhibits LIN-28A through the Activation of miRNA-98 in the Lung Cancer Cell Line A549.
Application : WB
Reactivity : Human
You BJ et al. Chem Res Toxicol 2019; 32 (2) : 255-264
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169