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ACOT9 antibody [N1C1]

Anti-ACOT9 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115724
Anti-ACOT9 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX115724
Anti-ACOT9 antibody [N1C1] used in Western Blot (WB). GTX115724

Cat. No. GTX115724

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ACOT9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACOT9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171015

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acyl-CoA thioesterase 9 , ACATE2 , CGI-16 , MT-ACT48 , MTACT48

Cellular Localization

Mitochondrion

Background

The protein encoded by this gene is a mitochondrial acyl-CoA thioesterase of unknown function. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ACOT9 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115724

GTX115724 ICC/IF Image

ACOT9 antibody [N1C1] detects ACOT9 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ACOT9 protein stained by ACOT9 antibody [N1C1] (GTX115724) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ACOT9 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX115724

GTX115724 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ACOT9(GTX115724) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ACOT9 antibody [N1C1] used in Western Blot (WB). GTX115724

GTX115724 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
10% SDS PAGE
GTX115724 diluted at 1:500

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169