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ACOX3 antibody

Anti-ACOX3 antibody used in Western Blot (WB). GTX115077
Anti-ACOX3 antibody used in Western Blot (WB). GTX115077
Anti-ACOX3 antibody used in IHC (Paraffin sections) (IHC-P). GTX115077

Cat No. GTX115077

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

A549 , mouse liver

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ACOX3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619796

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

acyl-CoA oxidase 3, pristanoyl

Cellular Localization

Peroxisome

Background

Acyl-Coenzyme A oxidase 3 also know as pristanoyl -CoA oxidase (ACOX3)is involved in the desaturation of 2-methyl branched fatty acids in peroxisomes. Unlike the rat homolog, the human gene is expressed in very low amounts in liver such that its mRNA was undetectable by routine Northern-blot analysis or its product by immunoblotting or by enzyme activity measurements. However the human cDNA encoding a 700 amino acid protein with a peroxisomal targeting C-terminal tripeptide S-K-L was isolated and is thought to be expressed under special conditions such as specific developmental stages or in a tissue specific manner in tissues that have not yet been examined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ACOX3 antibody used in Western Blot (WB). GTX115077

GTX115077 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX115077 diluted at 1:1000

Anti-ACOX3 antibody used in Western Blot (WB). GTX115077

GTX115077 WB Image

ACOX3 antibody detects ACOX3 protein by Western blot analysis.
A. 50 μg mouse liver lysate/extract
7.5 % SDS-PAGE
ACOX3 antibody (GTX115077) dilution: 1:500

Anti-ACOX3 antibody used in IHC (Paraffin sections) (IHC-P). GTX115077

GTX115077 IHC-P Image

ACOX3 antibody detects ACOX3 protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
ACOX3 antibody (GTX115077) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169