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ACP2 antibody [N2C3]

Anti-ACP2 antibody [N2C3] used in Western Blot (WB). GTX114234
Anti-ACP2 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114234
Anti-ACP2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114234

Cat No. GTX114234

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , *H3347 , *CX-1 , *SW48 , *HCT116 , *Human Lymphoma Tissue

Predict Reactivity

Mouse, Rat, Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620668

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

acid phosphatase 2, lysosomal , LAP

Cellular Localization

Lysosome membrane; Single-pass membrane protein; Lumenal side , Lysosome lumen

Background

This gene encodes the beta subunit of lysosomal acid phosphatase (LAP). LAP is chemically and genetically distinct from red cell acid phosphatase. The encoded protein belongs to a family of distinct isoenzymes which hydrolyze orthophosphoric monoesters to alcohol and phosphate. Mutations in this gene or in the related alpha subunit gene cause acid phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ACP2 antibody [N2C3] used in Western Blot (WB). GTX114234

GTX114234 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
Lysosomal acid phosphatase2 antibody
GTX114234 diluted at 1:1000

Anti-ACP2 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114234

GTX114234 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using ACP2(GTX114234) antibody at 1:200 dilution.

Anti-ACP2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114234

GTX114234 IHC-P Image

ACP2 antibody [N2C3] detects ACP2 protein at lysosome on Ca922 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Ca922 xenograft.
ACP2 antibody [N2C3] (GTX114234) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for ACP2 antibody [N2C3] (GTX114234). Be the first to share your publications with this product.

REVIEW

ACP2 antibody [N2C3] Cat No. GTX114234

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 03-Jul-2012
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : lymphoma tissue
Fixation : Formalin
Permeabilization /Antigen retrieval : iVIEW CC1 (Tris, EDTA, pH8.5)
Primary Antibody : 1:400, 37°C, 1Hr
Date : Anonymous submitted on 19-Jun-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : H3347 cells, CX-1 cells,SW48 cells,HCT116 cells
Amount used : 45μg
Blocking : 5% skim milk in PBS, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169