ACP2 antibody [N2C3]

Name: ACP2 antibody [N2C3]
Brand: GeneTex
SKU: GTX114234
Price: 399.00 USD
Rating: 5 (2 reviews)

Anti-ACP2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114234

Anti-ACP2 antibody [N2C3] used in Western Blot (WB). GTX114234

Anti-ACP2 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114234

Cat. No. GTX114234

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all ACP2 products

Datasheet
Document

View product citations for antibody GTX114234 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Mouse, Rat, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620668

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acid phosphatase 2, lysosomal , LAP

Cellular Localization

Lysosome membrane; Single-pass membrane protein; Lumenal side , Lysosome lumen

Background

This gene encodes the beta subunit of lysosomal acid phosphatase (LAP). LAP is chemically and genetically distinct from red cell acid phosphatase. The encoded protein belongs to a family of distinct isoenzymes which hydrolyze orthophosphoric monoesters to alcohol and phosphate. Mutations in this gene or in the related alpha subunit gene cause acid phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]

Database

Gene ID: 53 ACP2
UniProt: P11117 ACP2

Research Area

DATA IMAGES

GTX114234 IHC-P Image

ACP2 antibody [N2C3] detects ACP2 protein at lysosome on Ca922 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Ca922 xenograft.
ACP2 antibody [N2C3] (GTX114234) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114234 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
Lysosomal acid phosphatase2 antibody
GTX114234 diluted at 1:1000

GTX114234 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using ACP2(GTX114234) antibody at 1:200 dilution.

REFERENCE

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REVIEW