APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human ACSL1.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
acyl-CoA synthetase long chain family member 1 , ACS1 , FACL1 , FACL2 , LACS , LACS1 , LACS2
Cellular Localization
Mitochondrion outer membrane,Endoplasmic reticulum membrane
Background
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
Database
Research Area
DATA IMAGES
![Anti-ACSL1 antibody [HL1651] used in Western Blot (WB). GTX637232](/templates/default/js/lightbox/images/loading.gif "Anti-ACSL1 antibody [HL1651] used in Western Blot (WB). GTX637232")
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GTX637232 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACSL1 antibody [HL1651] (GTX637232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637232 WB Image
Untreated and treated 3T3-L1 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACSL1 antibody [HL1651] (GTX637232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637232 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACSL1 antibody [HL1651] (GTX637232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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|
GTX637232 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACSL1 antibody [HL1651] (GTX637232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX637232 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACSL1 antibody [HL1651] (GTX637232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for ACSL1 antibody [HL1651] (GTX637232). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ACSL1 antibody [HL1651] (GTX637232). Be the first to share your experience with this product.
![Anti-ACSL1 antibody [HL1651] used in Western Blot (WB). GTX637232](/upload/website/prouct_img/small/GTX637232/GTX637232_T-44746_20231027_WB_shRNA_watermark_23103019_561.webp "Anti-ACSL1 antibody [HL1651] used in Western Blot (WB). GTX637232")
![Anti-ACSL1 antibody [HL1651] used in Western Blot (WB). GTX637232](/upload/website/prouct_img/small/GTX637232/GTX637232_T-44746_20231110_WB_M_Differentiated_23111422_936.webp "Anti-ACSL1 antibody [HL1651] used in Western Blot (WB). GTX637232")
![Anti-ACSL1 antibody [HL1651] used in Western Blot (WB). GTX637232](/upload/website/prouct_img/small/GTX637232/GTX637232_T-45278_20240301_WB_R_tissue_24030600_283.webp "Anti-ACSL1 antibody [HL1651] used in Western Blot (WB). GTX637232")
![Anti-ACSL1 antibody [HL1651] used in Western Blot (WB). GTX637232](/upload/website/prouct_img/small/GTX637232/GTX637232_T-45278_20240301_WB_TPM_watermark_24030600_488.webp "Anti-ACSL1 antibody [HL1651] used in Western Blot (WB). GTX637232")
![Anti-ACSL1 antibody [HL1651] used in Western Blot (WB). GTX637232](/upload/website/prouct_img/small/GTX637232/GTX637232_T-45278_20240301_WB_M_tissue_24030600_911.webp "Anti-ACSL1 antibody [HL1651] used in Western Blot (WB). GTX637232")
![Anti-ACSL1 antibody [HL1651] used in Western Blot (WB). GTX637232](/upload/website/prouct_img/normal/GTX637232/GTX637232_T-44746_20231027_WB_shRNA_watermark_23103019_561.webp "Anti-ACSL1 antibody [HL1651] used in Western Blot (WB). GTX637232")
![Anti-ACSL1 antibody [HL1651] used in Western Blot (WB). GTX637232](/upload/website/prouct_img/normal/GTX637232/GTX637232_T-44746_20231110_WB_M_Differentiated_23111422_936.webp "Anti-ACSL1 antibody [HL1651] used in Western Blot (WB). GTX637232")
![Anti-ACSL1 antibody [HL1651] used in Western Blot (WB). GTX637232](/upload/website/prouct_img/normal/GTX637232/GTX637232_T-45278_20240301_WB_R_tissue_24030600_283.webp "Anti-ACSL1 antibody [HL1651] used in Western Blot (WB). GTX637232")
![Anti-ACSL1 antibody [HL1651] used in Western Blot (WB). GTX637232](/upload/website/prouct_img/normal/GTX637232/GTX637232_T-45278_20240301_WB_TPM_watermark_24030600_488.webp "Anti-ACSL1 antibody [HL1651] used in Western Blot (WB). GTX637232")
![Anti-ACSL1 antibody [HL1651] used in Western Blot (WB). GTX637232](/upload/website/prouct_img/normal/GTX637232/GTX637232_T-45278_20240301_WB_M_tissue_24030600_911.webp "Anti-ACSL1 antibody [HL1651] used in Western Blot (WB). GTX637232")