APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.2-2.5 ug/ml |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
MCF-7
PROPERTIES
Form
Liquid
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to a N-terminal region of Human ACSL1
Purification
Affinity Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
acyl-CoA synthetase long chain family member 1 , ACS1 , FACL1 , FACL2 , LACS , LACS1 , LACS2
Cellular Localization
Mitochondrion outer membrane,Endoplasmic reticulum membrane
Background
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
Database
Research Area
DATA IMAGES
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GTX47781 WB Image
WB analysis of MCF-7 cells using GTX47781 ACSL1 antibody at 0.2-1μg/ml.
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GTX47781 ICC/IF Image
ICC/IF analysis of THP-1 cells using GTX47781 ACSL1 antibody at 1:200. Green : ASCL1, Red : Bodipy (stain for netural lipids).
THP-1 cells was treated with 20mM PMA for 24hrs and then activated by culturing in the presence of irradiated M. tuberculosis at 0.050 mg/ml. for 3 days. Cells were fixed with 4% PFA, washed and stained with primary and then secondary antibody in blocking buffer containing 5% human serum.
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REFERENCE
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REVIEW
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