GeneTex
United States (US)

ACSL6 antibody

GTX115693_40296_WB_18121410_277.jpg
GTX115693_40296_WB_R_heart_18121410_654.jpg
GTX115693_40296_20141205_IHC_18121410_219.jpg
GTX115693_40296_WB_M_heart_18121410_459.jpg

Cat No. GTX115693

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , THP-1 , NCI-H929 , mouse heart , rat heart

Predict Reactivity

Rabbit, Bovine, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ACSL6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Acyl-Coa Synthetase Long Chain Family Member 6,Acs2,Facl6,Lacs 6,Lacs2,Lacs5,Acsl6

Cellular Localization

Mitochondrion outer membrane; Single-pass type III membrane protein , Peroxisome membrane; Single-pass type III membrane protein , Microsome membrane; Single-pass type III membrane protein , Endoplasmic reticulum membrane; Single-pass type III membrane pr

Background

Long-chain acyl-CoA synthetases (EC 6.2.1.3), such as ACSL6, catalyze the formation of acyl-CoA from fatty acids, ATP, and CoA (Malhotra et al., 1999 [PubMed 10548543]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX115693_40296_WB_18121410_277.jpg

GTX115693 WB Image

ACSL6 antibody detects ACSL6 protein by Western blot analysis.
A. 30 µg Jurkat whole cell lysate/extract
B. 30 µg Raji whole cell lysate/extract
C. 30 µg K562 whole cell lysate/extract
D. 30 µg THP-1 whole cell lysate/extract
E. 30 µg NCI-H929 whole cell lysate/extract
7.5 % SDS-PAGE
ACSL6 antibody (GTX115693) dilution: 1:1000

GTX115693_40296_WB_R_heart_18121410_654.jpg

GTX115693 WB Image

ACSL6 antibody detects ACSL6 protein by Western blot analysis.
A. 50 µg rat heart lysate/extract
7.5 % SDS-PAGE
ACSL6 antibody (GTX115693) dilution: 1:10000

GTX115693_40296_20141205_IHC_18121410_219.jpg

GTX115693 IHC-P Image

ACSL6 antibody detects ACSL6 protein at cytoplasm on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatoma.
ACSL6 antibody (GTX115693) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115693_40296_WB_M_heart_18121410_459.jpg

GTX115693 WB Image

ACSL6 antibody detects ACSL6 protein by Western blot analysis.
A. 50 µg mouse heart lysate/extract
7.5 % SDS-PAGE
ACSL6 antibody (GTX115693) dilution: 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf