GeneTex
United States (US)

ACSM1 antibody

GTX123303_40730_IHC_18121410_785.jpg
GTX123303_40730_WB_18121410_402.jpg

Cat No. GTX123303

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

Jurkat , Raji , THP-1
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACSM1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Acyl-Coa Synthetase Medium Chain Family Member 1,Bucs1,Macs1,Acsm1

Cellular Localization

Mitochondrion matrix

Background

Has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C(4) to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids (in vitro). Functions as GTP-dependent lipoate-activating enzyme that generates the substrate for lipoyltransferase.

Database

Research Area

DATA IMAGES
GTX123303_40730_IHC_18121410_785.jpg

GTX123303 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ACSM1(GTX123303) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX123303_40730_WB_18121410_402.jpg

GTX123303 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: THP-1
7.5% SDS PAGE
GTX123303 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169