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ACSS2 antibody

Orthogonal
Anti-ACSS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX30020
Anti-ACSS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX30020
Anti-ACSS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30020
Anti-ACSS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30020
Anti-ACSS2 antibody used in Western Blot (WB). GTX30020

Cat. No. GTX30020

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($399)
See all ACSS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein of human ACSS2

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887586

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acyl-CoA synthetase short chain family member 2 , ACAS2 , ACECS , ACS , ACSA , dJ1161H23.1

Cellular Localization

Cytoplasm

Background

This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]

Database

Research Area

DATA IMAGES

Anti-ACSS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX30020

GTX30020 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX30020 ACSS2 antibody.
Dilution : 1:100

Anti-ACSS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX30020

GTX30020 IHC-P Image

IHC-P analysis of rat heart tissue using GTX30020 ACSS2 antibody.
Dilution : 1:100

Anti-ACSS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30020

GTX30020 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX30020 ACSS2 antibody.
Blue : DAPI
Dilution : 1:100

Anti-ACSS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30020

GTX30020 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX30020 ACSS2 antibody.
Blue : DAPI
Dilution : 1:200

Anti-ACSS2 antibody used in Western Blot (WB). GTX30020

GTX30020 WB Image

WB analysis of various sample lysates using GTX30020 ACSS2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

Application Reference

Tsao CH et al. Front Med (Lausanne) 2022; 9 : 1008200 Dual-tracer positron emission tomography/computed tomography as an imaging probe of de novo lipogenesis in preclinical models of hepatocellular carcinoma.
Application : WB
Reactivity : Human
Li X et al. Mol Cell 2017; 66 (5) : 684-697.e9 Nucleus-Translocated ACSS2 Promotes Gene Transcription for Lysosomal Biogenesis and Autophagy.
Application : WB, ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399