GeneTex
United States (US)

ACTL8 antibody

GTX106696_WB_19011506_626.jpg
GTX106696_40135_20150529_IHC_19011506_277.jpg
GTX106696_40135_IFA_19011506_101.jpg

Cat No. GTX106696

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , Molt-4
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACTL8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CT57 antibody, ACTL8 antibody, actin like protein antibody, actin-like protein 8 antibody, cancer/testis antigen 57 antibody, actin-like 8 antibody

Cellular Localization

Cytoplasm , cytoskeleton

Database

DATA IMAGES
GTX106696_WB_19011506_626.jpg

GTX106696 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
B: Molt-4 (GTX27912)
10% SDS PAGE
GTX106696 diluted at 1:1000

GTX106696_40135_20150529_IHC_19011506_277.jpg

GTX106696 IHC-P Image

ACTL8 antibody detects ACTL8 protein at cytoplasm in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
ACTL8 antibody (GTX106696) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX106696_40135_IFA_19011506_101.jpg

GTX106696 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using ACTL8(GTX106696) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169