GeneTex
United States (US)

ACTR1A antibody

GTX115348_40422_WB_18121410_960.jpg
GTX115348_40422_WB_M_liver_18121410_549.jpg
GTX115348_40422_IHC_18121410_939.jpg

Cat No. GTX115348

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

293T , mouse liver

Predict Reactivity

Rat, Bovine, Dog, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACTR1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Arp1 Actin Related Protein 1 Homolog A,Arp1,Arp1A,Ctrn1,Actr1A

Cellular Localization

Cytoplasm , cytoskeleton , Centrosome

Background

This gene encodes a 42.6 kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 8-13 copies per dynactin molecule, and is the most abundant molecule in the dynactin complex. It is an actin-related protein, and is approximately 60% identical at the amino acid level to conventional actin. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115348_40422_WB_18121410_960.jpg

GTX115348 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX115348 diluted at 1:2000

GTX115348_40422_WB_M_liver_18121410_549.jpg

GTX115348 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX115348 diluted at 1:1000

GTX115348_40422_IHC_18121410_939.jpg

GTX115348 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung cancer, using ACTR1A(GTX115348) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169