APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , Mouse liver
Predict Reactivity
Rat, Bovine, Dog, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.65 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ACTR1A. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10720433
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
actin related protein 1A , ARP1 , Arp1A , CTRN1
Cellular Localization
Cytoplasm , cytoskeleton , Centrosome
Background
This gene encodes a 42.6 kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 8-13 copies per dynactin molecule, and is the most abundant molecule in the dynactin complex. It is an actin-related protein, and is approximately 60% identical at the amino acid level to conventional actin. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX115348 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human lung cancer, using ACTR1A(GTX115348) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX115348 WB Image
Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX115348 diluted at 1:1000
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GTX115348 WB Image
Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX115348 diluted at 1:2000
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REFERENCE
There are currently no references for ACTR1A antibody (GTX115348). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ACTR1A antibody (GTX115348). Be the first to share your experience with this product.
