APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
IMR32 , Mouse brain
Predict Reactivity
Rat, Zebrafish, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.69 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ACTR1B. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10724591
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
actin related protein 1B , ARP1B , CTRN2 , PC3
Cellular Localization
Cytoplasm , cytoskeleton , Centrosome
Background
This gene encodes a 42.3 kD subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein and is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like ACTR1A, is an actin-related protein. These two proteins, which are of equal length and share 90% amino acid identity, are present in a constant ratio of approximately 1:15 in the dynactin complex. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX115347 IHC-P Image
ACTR1B antibody detects ACTR1B protein at cytosol on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded ovarian carcinoma.
ACTR1B antibody (GTX115347) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX115347 WB Image
Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX115347 diluted at 1:1000
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GTX115347 ICC/IF Image
ACTR1B antibody detects ACTR1B protein at cytoskeleton by immunofluorescent analysis.
Sample: SKNSH cells were fixed in ice-cold MeOH for 5 min.
Green: ACTR1B protein stained by ACTR1B antibody (GTX115347) diluted at 1:1000.
Blue: Hoechst 33342 staining.
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GTX115347 WB Image
Sample (30 ug of whole cell lysate)
A: IMR32
10% SDS PAGE
GTX115347 diluted at 1:1000
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REFERENCE
There are currently no references for ACTR1B antibody (GTX115347). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ACTR1B antibody (GTX115347). Be the first to share your experience with this product.
