GeneTex
United States (US)

ACTR1B antibody

GTX115347_40415_WB_M_brain_18121410_887.jpg
GTX115347_40415_WB_18121410_671.jpg
GTX115347_40415_IHC_18121410_664.jpg
GTX115347_40415_IFA_18121410_511.jpg

Cat No. GTX115347

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

IMR32 , mouse brain

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACTR1B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Arp1 Actin Related Protein 1 Homolog B,Arp1B,Ctrn2,Pc3,Actr1B

Cellular Localization

Cytoplasm , cytoskeleton , Centrosome

Background

This gene encodes a 42.3 kD subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein and is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like ACTR1A, is an actin-related protein. These two proteins, which are of equal length and share 90% amino acid identity, are present in a constant ratio of approximately 1:15 in the dynactin complex. [provided by RefSeq]

Database

DATA IMAGES
GTX115347_40415_WB_M_brain_18121410_887.jpg

GTX115347 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX115347 diluted at 1:1000

GTX115347_40415_WB_18121410_671.jpg

GTX115347 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
10% SDS PAGE
GTX115347 diluted at 1:1000

GTX115347_40415_IHC_18121410_664.jpg

GTX115347 IHC-P Image

ACTR1B antibody detects ACTR1B protein at cytosol on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded ovarian carcinoma.
ACTR1B antibody (GTX115347) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115347_40415_IFA_18121410_511.jpg

GTX115347 ICC/IF Image

ACTR1B antibody detects ACTR1B protein at cytoskeleton by immunofluorescent analysis.
Sample: SKNSH cells were fixed in ice-cold MeOH for 5 min.
Green: ACTR1B protein stained by ACTR1B antibody (GTX115347) diluted at 1:1000.
Blue: Hoechst 33342 staining.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169