APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-0.5μg/ml |
| 10μg/ml |
| Assay dependent |
|
Note :
ICC/IF
Strong expression of the protein seen in the cytoplasm and nucleus of U2OS cells.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89580-PEP).
Product Note
This antibody is expected to recognise isoform NP_000656 only (the ubiquitously expressed, hydrophillic form).
Predict Reactivity
Mouse(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence QFDHYSKQDRCSDL, from the C Terminus of the protein sequence according to NP_000656.1; NP_001269378.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
acetylcholinesterase (Cartwright blood group) , ACEE , ARACHE , N-ACHE , YT
Cellular Localization
Cell junction, synapse , Secreted , Cell membrane
Background
Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX89580 WB Image
WB analysis of rat brain lysate using GTX89580 AChE antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89580 WB Image
WB analysis of Daudi (A), HeLa (B) and Jurkat (C) cell lysate using GTX89580 AChE antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89580 FACS Image
FACS analysis of PFA fixed HeLa cells using GTX89580 AChE antibody, C-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 1μg/ml
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GTX89580 FACS Image
FACS analysis of PFA fixed HeLa cells using GTX89580 AChE antibody, C-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml
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GTX89580 WB Image
WB analysis of HepG2 (A), Daudi (B) and HeLa (C) and Jurkat (D) cell lysate (35μg protein in RIPA buffer) using GTX89580 AChE antibody, C-term.
Dilution : 1μg/ml (HepG2 and Daudi) and 0.3μg/ml (HeLa and Jurkat)
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GTX89580 ICC/IF Image
ICC/IF analysis of PFA fixed U2OS cells using GTX89580 AChE antibody, C-term.
Green : Primary antibody
Blue : DAPI
Permeabilization : 0.15% Triton
Dilution : 10μg/ml
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REFERENCE
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REVIEW
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