ICC/IF analysis of PFA fixed U2OS cells using GTX89580 AChE antibody, C-term.
Green : Primary antibody
Blue : DAPI
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

WB analysis of HepG2 (A), Daudi (B) and HeLa (C) and Jurkat (D) cell lysate (35μg protein in RIPA buffer) using GTX89580 AChE antibody, C-term.
Dilution : 1μg/ml (HepG2 and Daudi) and 0.3μg/ml (HeLa and Jurkat)

FACS analysis of PFA fixed HeLa cells using GTX89580 AChE antibody, C-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml

FACS analysis of PFA fixed HeLa cells using GTX89580 AChE antibody, C-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 1μg/ml

WB analysis of Daudi (A), HeLa (B) and Jurkat (C) cell lysate using GTX89580 AChE antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

WB analysis of rat brain lysate using GTX89580 AChE antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer