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AChE antibody [HR2]

Anti-AChE antibody [HR2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22803
Anti-AChE antibody [HR2] used in IHC (Paraffin sections) (IHC-P). GTX22803
Anti-AChE antibody [HR2] used in IHC (Paraffin sections) (IHC-P). GTX22803
Anti-AChE antibody [HR2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22803
Anti-AChE antibody [HR2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22803
Anti-AChE antibody [HR2] used in IHC (Paraffin sections) (IHC-P). GTX22803

Cat. No. GTX22803

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 HR2

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, IHC

Reactivity

 Human, Mouse, Rabbit, Bovine, Cat, Guinea pig
Package
200 μl ($399)
See all AChE products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr 1:50
IP Assay dependent
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Product Note

This antibody does not cross react with rat or frog AChE, and does not detect butyrylcholinesterase (BChE). Immunohistochemical staining of AChE in human brain samples with GTX22803 results in staining of nerve fibers and terminals.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified human cerebellar acetylcholinesterase.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_384860

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acetylcholinesterase (Cartwright blood group) , ACEE , ARACHE , N-ACHE , YT

Cellular Localization

Cell junction, synapse,Secreted,Cell membrane

Background

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-AChE antibody [HR2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22803

GTX22803 ICC/IF Image

ICC/IF analysis of Neuro-2a Cells using GTX22803 AChE antibody [HR2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-AChE antibody [HR2] used in IHC (Paraffin sections) (IHC-P). GTX22803

GTX22803 IHC-P Image

IHC-P analysis of human rectum tissue using GTX22803 AChE antibody [HR2].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-AChE antibody [HR2] used in IHC (Paraffin sections) (IHC-P). GTX22803

GTX22803 IHC-P Image

IHC-P analysis of human Brain tissue using GTX22803 AChE antibody [HR2].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-AChE antibody [HR2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22803

GTX22803 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22803 AChE antibody [HR2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-AChE antibody [HR2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22803

GTX22803 ICC/IF Image

ICC/IF analysis of U251 cells using GTX22803 AChE antibody [HR2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-AChE antibody [HR2] used in IHC (Paraffin sections) (IHC-P). GTX22803

GTX22803 IHC-P Image

IHC-P analysis of human cerebellum tissue using GTX22803 AChE antibody [HR2].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50

REFERENCE

Application Reference

Jean L et al. J Biol Chem 2019; (Epub) In vivo localization of human acetylcholinesterase-derived species in a β-sheet conformation at the core of senile plaques in Alzheimer's disease.
Dafferner AJ et al. Chem Res Toxicol 2017; (Epub) Immunopurification of acetylcholinesterase from red blood cells for detection of nerve agent exposure.
Application : IHC-P, IP, ELISA
Reactivity : Human
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399